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Literature summary for 2.7.1.156 extracted from
- The biosynthesis of adenosylcobalamin (vitamin B12) (2002), Nat. Prod. Rep., 19, 390-412.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Salmonella enterica |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20000 | - |
amino acid sequence, small polypeptide with 180 amino acids | Salmonella enterica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + adenosylcobinamide | Escherichia coli | cobalamin biosynthesis, involved in de novo synthesis of vitamin B12 | adenosylcobinamide phosphate + ADP | - |
? |  |
| ATP + adenosylcobinamide | Priestia megaterium | cobalamin biosynthesis, involved in de novo synthesis of vitamin B12 | adenosylcobinamide phosphate + ADP | - |
? | |
| ATP + adenosylcobinamide | Pseudomonas denitrificans (nom. rej.) | cobalamin biosynthesis, involved in de novo synthesis of vitamin B12 | adenosylcobinamide phosphate + ADP | - |
? | |
| ATP + adenosylcobinamide | Propionibacterium freudenreichii | cobalamin biosynthesis, involved in de novo synthesis of vitamin B12 | adenosylcobinamide phosphate + ADP | - |
? | |
| ATP + adenosylcobinamide | Salmonella enterica | cobalamin biosynthesis, involved in de novo synthesis of vitamin B12, uses GTP to guanylate itself, then transfers this GMP group to AdoCbi-P to yield AdoCbi-GDP, the final product of the reaction, CobU enzyme in vivo is always guanylated, guanylation is necessary for both kinase and guanyltransferase activity | adenosylcobinamide phosphate + ADP | - |
? | |
| GTP + adenosylcobinamide | Escherichia coli | - |
adenosylcobinamide phosphate + GDP | - |
? | |
| GTP + adenosylcobinamide | Priestia megaterium | - |
adenosylcobinamide phosphate + GDP | - |
? | |
| GTP + adenosylcobinamide | Pseudomonas denitrificans (nom. rej.) | - |
adenosylcobinamide phosphate + GDP | - |
? | |
| GTP + adenosylcobinamide | Salmonella enterica | - |
adenosylcobinamide phosphate + GDP | - |
? | |
| GTP + adenosylcobinamide | Propionibacterium freudenreichii | - |
adenosylcobinamide phosphate + GDP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| no activity in archaebacteria | - |
- |
- |
| no activity in eukaryota | - |
- |
- |
| Priestia megaterium | - |
- |
- |
| Propionibacterium freudenreichii | - |
formerly Propionibacterium shermanii | - |
| Pseudomonas denitrificans (nom. rej.) | - |
- |
- |
| Salmonella enterica | - |
serovar typhimurium | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2',5'-dideoxyadenosylcobinamide | highest kinase activity | Pseudomonas denitrificans (nom. rej.) | 2',5'-dideoxyadenosylcobinamide phosphate + ADP | - |
? | |
| ATP + 2',5'-dideoxyadenosylcobinamide | highest kinase activity | Salmonella enterica | 2',5'-dideoxyadenosylcobinamide phosphate + ADP | - |
? | |
| ATP + 5'-deoxyadenosylcobinamide | highest kinase activity | Pseudomonas denitrificans (nom. rej.) | 5'-deoxyadenosylcobinamide phosphate + ADP | - |
? | |
| ATP + 5'-deoxyadenosylcobinamide | highest kinase activity | Salmonella enterica | 5'-deoxyadenosylcobinamide phosphate + ADP | - |
? | |
| ATP + 5'-deoxycytosylcobinamide | substantial amount of activity retains, about 65% of the activity with AdoCbi | Pseudomonas denitrificans (nom. rej.) | 5'-deoxycytosylcobinamide phosphate + ADP | - |
? | |
| ATP + 5'-deoxycytosylcobinamide | substantial amount of activity retains, about 65% of the activity with AdoCbi | Salmonella enterica | 5'-deoxycytosylcobinamide phosphate + ADP | - |
? | |
| ATP + 5'-deoxyguanosylcobinamide | substantial amount of activity retains, about 65% of the activity with AdoCbi | Pseudomonas denitrificans (nom. rej.) | 5'-deoxyguanosylcobinamide phosphate + ADP | - |
? | |
| ATP + 5'-deoxyguanosylcobinamide | substantial amount of activity retains, about 65% of the activity with AdoCbi | Salmonella enterica | 5'-deoxyguanosylcobinamide phosphate + ADP | - |
? | |
| ATP + 5'-deoxyinosylcobinamide | substantial amount of activity retains, about 65% of the activity with AdoCbi | Pseudomonas denitrificans (nom. rej.) | 5'-deoxyinosylcobinamide phosphate + ADP | - |
? | |
| ATP + 5'-deoxyinosylcobinamide | substantial amount of activity retains, about 65% of the activity with AdoCbi | Salmonella enterica | 5'-deoxyinosylcobinamide phosphate + ADP | - |
? | |
| ATP + adenosylcobinamide | - |
Escherichia coli | adenosylcobinamide phosphate + ADP | - |
? | |
| ATP + adenosylcobinamide | - |
Priestia megaterium | adenosylcobinamide phosphate + ADP | - |
? | |
| ATP + adenosylcobinamide | - |
Pseudomonas denitrificans (nom. rej.) | adenosylcobinamide phosphate + ADP | - |
? | |
| ATP + adenosylcobinamide | - |
Salmonella enterica | adenosylcobinamide phosphate + ADP | - |
? | |
| ATP + adenosylcobinamide | - |
Propionibacterium freudenreichii | adenosylcobinamide phosphate + ADP | - |
? | |
| ATP + adenosylcobinamide | cobalamin biosynthesis, involved in de novo synthesis of vitamin B12 | Escherichia coli | adenosylcobinamide phosphate + ADP | - |
? | |
| ATP + adenosylcobinamide | cobalamin biosynthesis, involved in de novo synthesis of vitamin B12 | Priestia megaterium | adenosylcobinamide phosphate + ADP | - |
? | |
| ATP + adenosylcobinamide | cobalamin biosynthesis, involved in de novo synthesis of vitamin B12 | Pseudomonas denitrificans (nom. rej.) | adenosylcobinamide phosphate + ADP | - |
? | |
| ATP + adenosylcobinamide | cobalamin biosynthesis, involved in de novo synthesis of vitamin B12 | Propionibacterium freudenreichii | adenosylcobinamide phosphate + ADP | - |
? | |
| ATP + adenosylcobinamide | cobalamin biosynthesis, involved in de novo synthesis of vitamin B12, uses GTP to guanylate itself, then transfers this GMP group to AdoCbi-P to yield AdoCbi-GDP, the final product of the reaction, CobU enzyme in vivo is always guanylated, guanylation is necessary for both kinase and guanyltransferase activity | Salmonella enterica | adenosylcobinamide phosphate + ADP | - |
? | |
| GTP + adenosylcobinamide | - |
Escherichia coli | adenosylcobinamide phosphate + GDP | - |
? | |
| GTP + adenosylcobinamide | - |
Priestia megaterium | adenosylcobinamide phosphate + GDP | - |
? | |
| GTP + adenosylcobinamide | - |
Pseudomonas denitrificans (nom. rej.) | adenosylcobinamide phosphate + GDP | - |
? | |
| GTP + adenosylcobinamide | - |
Salmonella enterica | adenosylcobinamide phosphate + GDP | - |
? | |
| GTP + adenosylcobinamide | - |
Propionibacterium freudenreichii | adenosylcobinamide phosphate + GDP | - |
? | |
| additional information | enzyme can bind CTP, UTP, and dCTP, but product formation is not observed, only ATP or GTP generate a catalytically competent conformation of the enzyme, no activity is observed if (CN)2Cbi is used as substrate | Salmonella enterica | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | - |
Salmonella enterica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| adenosylcobinamide kinase | - |
Escherichia coli |
| adenosylcobinamide kinase | - |
Priestia megaterium |
| adenosylcobinamide kinase | - |
Pseudomonas denitrificans (nom. rej.) |
| adenosylcobinamide kinase | - |
Salmonella enterica |
| adenosylcobinamide kinase | - |
Propionibacterium freudenreichii |
| CobP | - |
Pseudomonas denitrificans (nom. rej.) |
| CobU | - |
Escherichia coli |
| CobU | - |
Priestia megaterium |
| CobU | - |
Pseudomonas denitrificans (nom. rej.) |
| CobU | - |
Salmonella enterica |
| CobU | - |
Propionibacterium freudenreichii |
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