Literature summary for 2.7.1.159 extracted from

Abdullah, M.; Hughes, P.J.; Craxton, A.; Gigg, R.; Desai, T.; Marecek, J.F.; Prestwich, G.D.; Shears, S.B.
Purification and characterization of inositol-1,3,4-trisphosphate 5/6-kinase from rat liver using an inositol hexakisphosphate affinity column (1992), J. Biol. Chem., 267, 22340-22345.

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	enzyme activity is unaffected by protein kinase C, protein kinase A, and Ca2+/calmodulin	Rattus norvegicus

General Stability

General Stability	Organism
addition of 1 mM ATP at pH 6.1 stabilizes the enzyme during purification	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism	Structure
1D-myo-inositol-1,3,4,5-tetrakisphosphate	competitive	Rattus norvegicus
1D-myo-inositol-1,3,4,6-tetrakisphosphate	competitive	Rattus norvegicus
additional information	enzyme activity is unaffected by protein kinase C, protein kinase A, and Ca2+/calmodulin	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetics	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Rattus norvegicus	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
36000	-	x * 36000, SDS-PAGE	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
26171fold from liver by polyethylene glycol precipitation, ion exchange chromatography and heparin and inositol hexakisphosphate affinity chromatograpies	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1780	-	purified enzyme, determination of 5-kinase and 6-kinase activities	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 1D-myo-inositol-1,3,4-trisphosphate	5fold lower activity compared to 1D-myo-inositol-1,3,4,6-tetrakisphosphate formation	Rattus norvegicus	ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate	-	?
ATP + 1D-myo-inositol-1,3,4-trisphosphate	5fold higher activity compared to 1D-myo-inositol-1,3,4,5-tetrakisphosphate formation	Rattus norvegicus	ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 36000, SDS-PAGE	Rattus norvegicus

Synonyms

Synonyms	Comment	Organism
inositol-1,3,4-trisphosphate kinase	-	Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	assay at	Rattus norvegicus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Rattus norvegicus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.002	0.003	1D-myo-inositol-1,3,4,6-tetrakisphosphate	pH 7.2, 37°C, versus 1D-myo-inositol-1,3,4-trisphosphate	Rattus norvegicus
0.003	0.004	1D-myo-inositol-1,3,4,5-tetrakisphosphate	pH 7.2, 37°C, versus 1D-myo-inositol-1,3,4-trisphosphate	Rattus norvegicus

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Release 2023.1 (January 2023)