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Literature summary for 2.7.1.160 extracted from

  • Mc Craith, S.M.; Phizicky, E.M.
    An enzyme from Saccharomyces cerevisiae uses NAD+ to transfer the splice junction 2'-phosphate from ligated tRNA to an acceptor molecule (1991), J. Biol. Chem., 266, 11986-11992.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA inhibition of reaction by molar exess of EDTA over Mg2+ Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Saccharomyces cerevisiae
additional information Ca2+ and Zn2+ have no effect on activity Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-phospho-[ligated tRNA] + NAD+ specificity for substrates bearing an internal 2'-phosphate Saccharomyces cerevisiae mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
?

Cofactor

Cofactor Comment Organism Structure
additional information UMP, SAMP, cAMP, UTP, CTP, GTP, ATP and derivatives of NAD+ like NMN, ADP-ribose, ATP-ribose, NADPH, NADP+, NADH do not support dephosphorylation Saccharomyces cerevisiae
NAD+ heatstable Saccharomyces cerevisiae