Application | Comment | Organism |
---|---|---|
biotechnology | resistance against hyromycin B mediated by transformation of the hph gene | Escherichia coli |
molecular biology | resistance against hyromycin B mediated by transformation of the hph gene, a selectable marker gen. | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
in Colletotrichum falcatum and Colletotrichum acutatum. Agrobacterium tumefaciens-mediated transformation (ATMT) is used by using the vector pBHt2 that contains a t-DNA harboring the hygromycin B resistance gen (hygromycin phosphotransferase, hph) in the backbone of pCAMBIA1300. | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hygromycin B + ATP | Escherichia coli | Growth of Colletotrichum falcatum is completely inhibited at 50 microg/ml, whereas in Colletotrichum acutatum, a concentration of 300 microg/ml is most effective in inhibiting mycelial growth | 4-O-phosphohygromycin B + ADP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
The vector pBHt2 contains a T-DNA harboring hygromycin B resistance (hygromycin phosphotransferase, hph) in the backbone of pCAMBIA1300 descended from Escherichia coli, transformed in Colletrichum acutatum and Colletrichum falcatum via Agrobacterium tumefaciens | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | bacterial strain SK1044 | Escherichia coli | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hygromycin B + ATP | Growth of Colletotrichum falcatum is completely inhibited at 50 microg/ml, whereas in Colletotrichum acutatum, a concentration of 300 microg/ml is most effective in inhibiting mycelial growth | Escherichia coli | 4-O-phosphohygromycin B + ADP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HPH | - |
Escherichia coli |
hygromycin phosphotransferase | - |
Escherichia coli |