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Literature summary for 2.7.1.163 extracted from
- Tissue culture specificity of the tobacco ASA2 promoter driving hpt as a selectable marker for soybean transformation selection (2008), Plant Cell Rep., 27, 1705-1711.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | resistance against hyromycin B mediated by transformation of the hph gene | Escherichia coli |
| molecular biology | gene is used as selectable marker, mediates hygromycin resistence | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| The hpt coding region is obtained by a XhoI digestion of the vector pCAMBIA1380 and is cloned into the SalI-cut pBluescript KSII. The EcoRV-XhoI fragment of the hpt coding sequence is ligated with the SmaI-XhoI fragment of pHAG to obtain the intermediate plasmid pHyII, so that the hpt coding region is flanked by the 2.3 kb ASA2 promoter at the 5'end and the Arabidopsis actin2 terminator at the 3'end. Finally, the SacI-SacII fragment of pHyII is ligated into the SacI-SacII-cut pBluescript KSII to complete the transformation vector pXZIII-8. | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
Protein sequence is used (vector pCAMBIA1380) for a selectable marker for soybean transformation selection. | - |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HPT | - |
Escherichia coli |
| hygromycin phosphotransferase | - |
Escherichia coli |
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