Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | 50 mM NaN3, 10 mM phenylmethyl sulfonylfluoride, and H2O2 have no obvious effect on the enzymatic activity | Pyrococcus horikoshii |
Cloned (Comment) | Organism |
---|---|
- |
Pyrococcus horikoshii |
expressed in Escherichia coli | Pyrococcus horikoshii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuCl2 | 1 mM, 61% inhibition; 61% inhibition at 1 mM | Pyrococcus horikoshii | |
HgCl2 | 11% inhibition at 1 mM; 1 mM, 11% inhibition | Pyrococcus horikoshii | |
additional information | resistant to the urea, ethanol, 2-propanol, n-butanol and DMSO (10% v/v each) | Pyrococcus horikoshii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.044 | - |
D-glycerate | - |
Pyrococcus horikoshii | |
0.044 | - |
(R)-glycerate | the reaction mixture contains 1 mM (R)-glycerate, 1 mM ATP, 10 mM MgCl2, 0.3 mM NADH, 1 mM phosphoenolpyruvate, 10 nM purified glycerate kinase, 3.5 units pyruvate kinase and 5 units lactate dehydrogenase in 50 mM potassium phosphate (pH 7.0), at 45°C | Pyrococcus horikoshii | |
0.102 | - |
ATP | - |
Pyrococcus horikoshii | |
0.102 | - |
ATP | the reaction mixture contains 1 mM (R)-glycerate, 1 mM ATP, 10 mM MgCl2, 0.3 mM NADH, 1 mM phosphoenolpyruvate, 10 nM purified glycerate kinase, 3.5 units pyruvate kinase and 5 units lactate dehydrogenase in 50 mM potassium phosphate (pH 7.0), at 45°C | Pyrococcus horikoshii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 10 mM, 68% of the activity with Mg2+ | Pyrococcus horikoshii | |
K+ | 7.94fold increase of activity at 50 mM | Pyrococcus horikoshii | |
K+ | 50 mM, 7.9fold activation | Pyrococcus horikoshii | |
Li+ | 2.28fold increase of activity at 50 mM | Pyrococcus horikoshii | |
Li+ | 50 mM, 2.3fold activation | Pyrococcus horikoshii | |
Mg2+ | 10 mM, required for activity | Pyrococcus horikoshii | |
Mg2+ | no activity is observed in the absence of divalent metal ion and maximal activity is observed in the presence of Mg2+ (10 mM) | Pyrococcus horikoshii | |
Mn2+ | 10 mM, 76% of the activity with Mg2+ | Pyrococcus horikoshii | |
additional information | when Mn2+, Co2+, Ca2+, Sr2+ and Ni2+ is substituted for Mg2+, respectively, Mn2+, Co2+ and Ni2+ show 76, 68 and 11% activity of that for Mg2+ | Pyrococcus horikoshii | |
Na+ | 3.49fold increase of activity at 50 mM | Pyrococcus horikoshii | |
Na+ | 50 mM, 3.5fold activation | Pyrococcus horikoshii | |
NH4+ | 7.83fold increase of activity at 50 mM | Pyrococcus horikoshii | |
NH4+ | 50 mM, 7.8fold activation | Pyrococcus horikoshii | |
Ni2+ | 10 mM, 11% of the activity with Mg2+ | Pyrococcus horikoshii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
SDS-PAGE | Pyrococcus horikoshii |
47000 | - |
2 * 47000, SDS-PAGE | Pyrococcus horikoshii |
47400 | - |
calculated from amino acid sequence | Pyrococcus horikoshii |
50000 | - |
2 * 50000, gel filtration | Pyrococcus horikoshii |
100000 | - |
gel filtration | Pyrococcus horikoshii |
Organic Solvent | Comment | Organism |
---|---|---|
2-propanol | resistant to | Pyrococcus horikoshii |
DMSO | resistant to | Pyrococcus horikoshii |
Ethanol | resistant to | Pyrococcus horikoshii |
n-Butanol | resistant to | Pyrococcus horikoshii |
urea | resistant to | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | - |
- |
- |
Pyrococcus horikoshii | O58231 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity chromatography, HiTrap Q ion exchange chromatography, and SephacrylTM S-200 HR gel filtration | Pyrococcus horikoshii |
Storage Stability | Organism |
---|---|
90°C, 50 mM Tris-HCl and 100 mM NaCl buffer (pH 8.0), 12 h, almost no loss of activity | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + (R)-glycerate | at 32% of the activity with ATP | Pyrococcus horikoshii | AMP + 3-phospho-(R)-glycerate | - |
? | |
ADP + (R)-glycerate | 32% of the activity with ATP | Pyrococcus horikoshii | AMP + 2-phospho-(R)-glycerate | - |
? | |
ATP + (R)-glycerate | - |
Pyrococcus horikoshii | ADP + 3-phospho-(R)-glycerate | - |
? | |
ATP + (R)-glycerate | - |
Pyrococcus horikoshii | ADP + 2-phospho-(R)-glycerate | - |
? | |
ATP + D-glycerate | - |
Pyrococcus horikoshii | ADP + 2-phospho-D-glycerate | - |
? | |
CTP + (R)-glycerate | at 73% of the activity with ATP | Pyrococcus horikoshii | CDP + 3-phospho-(R)-glycerate | - |
? | |
CTP + (R)-glycerate | 73% of the activity with ATP | Pyrococcus horikoshii | CDP + 2-phospho-(R)-glycerate | - |
? | |
diphosphate + (R)-glycerate | at 112% of the activity with ATP | Pyrococcus horikoshii | phosphate + 3-phospho-(R)-glycerate | - |
? | |
diphosphate + (R)-glycerate | 112% of the activity with ATP | Pyrococcus horikoshii | phosphate + 2-phospho-(R)-glycerate | - |
? | |
GTP + (R)-glycerate | at 64% of the activity with ATP | Pyrococcus horikoshii | GDP + 3-phospho-(R)-glycerate | - |
? | |
GTP + (R)-glycerate | 64% of the activity with ATP | Pyrococcus horikoshii | GDP + 2-phospho-(R)-glycerate | - |
? | |
additional information | AMP is not a substrate | Pyrococcus horikoshii | ? | - |
? | |
UTP + (R)-glycerate | at 29% of the activity with ATP | Pyrococcus horikoshii | UDP + 3-phospho-(R)-glycerate | - |
? | |
UTP + (R)-glycerate | 29% of the activity with ATP | Pyrococcus horikoshii | UDP + 2-phospho-(R)-glycerate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 47000, SDS-PAGE | Pyrococcus horikoshii |
dimer | 2 * 50000, gel filtration | Pyrococcus horikoshii |
Synonyms | Comment | Organism |
---|---|---|
2-PGA forming glycerate kinase | - |
Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Pyrococcus horikoshii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | strong activity at 30-50°C | Pyrococcus horikoshii |
45 | 100 | 45°C: optimum, 100°C: about 50% of maximal activity | Pyrococcus horikoshii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
12 h, no loss of activity | Pyrococcus horikoshii |
100 | - |
about half of the maximal activity remains at 100°C | Pyrococcus horikoshii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
360 | - |
(R)-glycerate | the reaction mixture contains 1 mM (R)-glycerate, 1 mM ATP, 10 mM MgCl2, 0.3 mM NADH, 1 mM phosphoenolpyruvate, 10 nM purified glycerate kinase, 3.5 units pyruvate kinase and 5 units lactate dehydrogenase in 50 mM potassium phosphate (pH 7.0), at 45°C | Pyrococcus horikoshii | |
489.2 | - |
D-glycerate | - |
Pyrococcus horikoshii | |
500.6 | - |
ATP | - |
Pyrococcus horikoshii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Pyrococcus horikoshii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | 50% of maximal activity at pH 6.0 and 10.0 | Pyrococcus horikoshii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | half of the maximum activity remains at pH 6-10 | Pyrococcus horikoshii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Pyrococcus horikoshii |