Literature summary for 2.7.1.17 extracted from

Di Luccio, E.; Petschacher, B.; Voegtli, J.; Chou, H.T.; Stahlberg, H.; Nidetzky, B.; Wilson, D.K.
Structural and kinetic studies of induced fit in xylulose kinase from Escherichia coli (2007), J. Mol. Biol., 365, 783-798.

Search for more literature for 2.7.1.17

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapour diffusion method using 1.5 M ammonium sulfate, 50 mM sodium citrate, pH 6, 1% (w/v) t-butanol and 25% (v/v) ethylene glycol, at 25°C	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
D233A	loss of activity	Escherichia coli
D6A	19fold decreased ATPase activity compared to the wild type enzyme	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
5-fluoro-xylulose	0.18 mM, strong inhibition of the ATPase activity of xylulokinase	Escherichia coli
AMPPNP	-	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.29	-	D-xylulose	wild type enzyme	Escherichia coli
8.2	-	ATP	mutant D233A	Escherichia coli
14	-	D-ribulose	wild type enzyme	Escherichia coli
127	-	xylitol	wild type enzyme	Escherichia coli
141	-	D-arabitol	wild type enzyme	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55000	-	2 * 55000, dynamic light scattering	Escherichia coli
110000	-	dynamic light scattering	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P09099	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-arabitol	-	Escherichia coli	ADP + D-arabitol 5-phosphate	-	?
ATP + D-ribulose	-	Escherichia coli	ADP + D-ribulose 5-phosphate	-	?
ATP + D-xylulose	-	Escherichia coli	ADP + D-xylulose 5-phosphate	-	r
ATP + xylitol	-	Escherichia coli	ADP + ?	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 55000, dynamic light scattering	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0038	-	ATP	mutant D6A	Escherichia coli
0.13	-	ATP	mutant D233A	Escherichia coli
105	-	D-arabitol	wild type enzyme	Escherichia coli
235	-	D-ribulose	wild type enzyme	Escherichia coli
237	-	xylitol	wild type enzyme	Escherichia coli
255	-	D-xylulose	wild type enzyme	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Escherichia coli

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.026	-	5-fluoro-xylulose	in the presence of 5 mM ATP	Escherichia coli
0.15	-	5-fluoro-xylulose	in the presence of 0.167 mM ATP	Escherichia coli
0.71	-	AMPPNP	in the presence of 4.25 mM xylulose	Escherichia coli
1.1	-	AMPPNP	in the presence of 0.28 mM xylulose	Escherichia coli
1.9	-	AMPPNP	in the presence of 0.167 mM ATP	Escherichia coli

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)