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Literature summary for 2.7.1.170 extracted from

  • Uehara, T.; Suefuji, K.; Valbuena, N.; Meehan, B.; Donegan, M.; Park, J.T.
    Recycling of the anhydro-N-acetylmuramic acid derived from cell wall murein involves a two-step conversion to N-acetylglucosamine-phosphate (2005), J. Bacteriol., 187, 3643-3649.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.170

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
ADP strong inhibitor of AnmK Escherichia coli
additional information AnmK is not inhibited by N-acetyl-beta-muramate 6-phosphate Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
 ATP in 100 mM Tris-HCl (pH 7.5), 20 mM MgCl2, at 37°C Escherichia coli
1
-
 1,6-anhydro-N-acetyl-beta-muramate in 100 mM Tris-HCl (pH 7.5), 20 mM MgCl2, at 37°C Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Escherichia coli 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39500
-
 calculated from amino acid sequence Escherichia coli
40000
-
 SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli MG1655
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sephacel column chromatography, hydroxylapatite column chromatography, and Mono Q column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1,6-anhydro-N-acetyl-beta-muramate + H2O
-
 Escherichia coli ADP + N-acetyl-beta-muramate 6-phosphate + H+
-
 ?
ATP + 1,6-anhydro-N-acetyl-beta-muramate + H2O
-
 Escherichia coli MG1655 ADP + N-acetyl-beta-muramate 6-phosphate + H+
-
 ?
additional information AnmK does not phosphorylate N-acetyl-muramate Escherichia coli ?
-
 ?
additional information AnmK does not phosphorylate N-acetyl-muramate Escherichia coli MG1655 ?
-
 ?

Synonyms

Synonyms Comment Organism
anhMurNAc kinase
-
 Escherichia coli
anhydro-N-acetylmuramic acid kinase
-
 Escherichia coli
AnmK
-
 Escherichia coli
YdhH
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
 from no activity at pH 4.5 and 5% of the maximum activity at pH 6.0, the activity continues to increase to pH 10.0 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.4
-
 ADP in 100 mM Tris-HCl (pH 7.5), 20 mM MgCl2, at 37°C Escherichia coli