Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.170 extracted from

  • Bacik, J.P.; Whitworth, G.E.; Stubbs, K.A.; Yadav, A.K.; Martin, D.R.; Bailey-Elkin, B.A.; Vocadlo, D.J.; Mark, B.L.
    Molecular basis of 1,6-anhydro bond cleavage and phosphoryl transfer by Pseudomonas aeruginosa 1,6-anhydro-N-acetylmuramic acid kinase (2011), J. Biol. Chem., 286, 12283-12291.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.170

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Gold cells Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
AnmK in complex with 1,6-anhydro-N-acetylmuramic acid and ADP, hanging drop vapor diffusion method, using 25% (w/v) polyethylene glycol 4000, 0.2 M (NH4)2SO4, and 0.1 M tri-sodium citrate (pH 5.6) Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 1,6-anhydro-N-acetyl-beta-muramate in 50 mM HEPES, pH 7.5, 10 mM MgCl2, and 50 mM KCl, at 37°C Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9I5Q5
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA-agarose column chromatography and gel filtration Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1,6-anhydro-N-acetyl-beta-muramate + H2O
-
 Pseudomonas aeruginosa ADP + N-acetyl-beta-muramate 6-phosphate + H+
-
 ?
additional information AnmK is active only against 1,6-anhydro-N-acetyl-beta-muramate and not against 1,6-anhydro-N-acetylmuramyl peptides Pseudomonas aeruginosa ?
-
 ?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
1,6-anhydro-N-acetylmuramic acid kinase
-
 Pseudomonas aeruginosa
AnmK
-
 Pseudomonas aeruginosa