Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence comparisons | Streptococcus pyogenes |
Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type and selenomethionine-derivated epsilon2/zeta2 protein complexes, X-ray diffraction structure determination and analysis at 1.95 A and 3.1 A resolution, respectively, multiple anomalous diffraction, modeling | Streptococcus pyogenes |
Protein Variants | Comment | Organism |
---|---|---|
D67T | site-directed mutagenesis | Streptococcus pyogenes |
K46A | site-directed mutagenesis, the change in the Walker A motif enables cloning and expression of genes K46A and D67T in Escherichia coli without coexpression of the antagonistic gene epsilon | Streptococcus pyogenes |
K46A/D67T | site-directed mutagenesis, the change in the Walker A motif enables cloning and expression of genes K46A and D67T in Escherichia coli without coexpression of the antagonistic gene epsilon | Streptococcus pyogenes |
additional information | a mutant variant truncated after Val-234 lacks the helix K-loop-helix L appendage | Streptococcus pyogenes |
R158A | site-directed mutagenesis, the mutant construct can be cloned into Escherichia coli but not overexpressed | Streptococcus pyogenes |
R158A/R171S | site-directed mutagenesis, the mutant can be cloned and expressed | Streptococcus pyogenes |
R171S | site-directed mutagenesis | Streptococcus pyogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pyogenes | Q54944 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | free zeta acts as phosphotransferase by using ATP/GTP. In epsilon2zeta2, the toxin zeta is inactivated because the N-terminal helix of the antitoxin epsilon blocks the ATP/GTP-binding site, toxin-antitoxin interactions, overview | Streptococcus pyogenes | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
toxin zeta | - |
Streptococcus pyogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Streptococcus pyogenes | |
GTP | - |
Streptococcus pyogenes |
General Information | Comment | Organism |
---|---|---|
additional information | the antitoxin toxin system epsilon/zeta and antibiotic resistance proteins are encoded on the broad-host-range, low-copy-number plasmid pSM19035. Theepsilon2/zeta2 protein complex is biologically nontoxic. The predominant contacts between antitoxin epsilon and toxin zeta involve helix a of epsilon, toxin-antitoxin interactions, overview | Streptococcus pyogenes |