Literature summary for 2.7.1.181 extracted from

Hagelueken, G.; Huang, H.; Clarke, B.R.; Lebl, T.; Whitfield, C.; Naismith, J.H.
Structure of WbdD: a bifunctional kinase and methyltransferase that regulates the chain length of the O antigen in Escherichia coli O9a (2012), Mol. Microbiol., 86, 730-742.

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
2.2 A resolution structure reveals a bacterial methyltransferase domain joined to a eukaryotic kinase domain. The kinase domain is again fused to an extended C-terminal coiled-coil domain reminiscent of eukaryotic Myotonic Dystrophy Protein Kinase family kinases. Structures of co-complexes with two known eukaryotic protein kinase inhibitors which are potent inhibitors in vitro, but do not show any in vivo activity	Escherichia coli

Crystallization (Comment)

Organism

2.2 A resolution structure reveals a bacterial methyltransferase domain joined to a eukaryotic kinase domain. The kinase domain is again fused to an extended C-terminal coiled-coil domain reminiscent of eukaryotic Myotonic Dystrophy Protein Kinase family kinases. Structures of co-complexes with two known eukaryotic protein kinase inhibitors which are potent inhibitors in vitro, but do not show any in vivo activity

Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
D350A	88.8% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 95.2% with substrate D-mannose	Escherichia coli
D351A	0.9% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 1.1% with substrate D-mannose	Escherichia coli
D351E	4.5% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 1.5% with substrate D-mannose	Escherichia coli
DELTA398-418	6.7% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 4.7% with substrate D-mannose	Escherichia coli
E274A	45.4% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 66.6% with substrate D-mannose	Escherichia coli
H132A	complete loss of activity	Escherichia coli
H133A	complete loss of activity	Escherichia coli
N34D/Q35E/H197E	complete loss of activity	Escherichia coli
R203A	complete loss of activity	Escherichia coli
R270A	72.8% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 85.7% with substrate D-mannose	Escherichia coli
W355F	72.4% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 1.9% with substrate D-mannose	Escherichia coli
W355H	48.7% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 50.7% with substrate D-mannose	Escherichia coli
Y16F	complete loss of activity	Escherichia coli
Y230F	0.1% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 2.5% with substrate D-mannose	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	J7I4B7	-	-
Escherichia coli O9a	J7I4B7	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ATP + 2-alpha-D-mannosyl-D-mannose	-	Escherichia coli	ADP + 3-O-phospho-2-alpha-D-mannosyl-D-mannose	-	?
ATP + 2-alpha-D-mannosyl-D-mannose	-	Escherichia coli O9a	ADP + 3-O-phospho-2-alpha-D-mannosyl-D-mannose	-	?
ATP + D-mannose	-	Escherichia coli	ADP + 3-O-phospho-D-mannose	-	?
ATP + D-mannose	-	Escherichia coli O9a	ADP + 3-O-phospho-D-mannose	-	?