Application | Comment | Organism |
---|---|---|
synthesis | isobutene production rates are 507 pmol/min*g cells using Escherichia coli cells expressing the enzyme and 2880 pmol/min*mg protein with the purified histidine-tagged enzyme. Isobutene is a small, highly reactive molecule, used extensively as a platform chemical to manufacture a wide variety of products including fuel additives, rubbers, and speciality chemicals | Picrophilus torridus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Picrophilus torridus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
ATP | pH 7.5, 30°C | Picrophilus torridus | |
0.131 | - |
(R)-mevalonate | pH 7.5, 30°C | Picrophilus torridus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-mevalonate | Picrophilus torridus | key enzyme of the Thermoplasma acidophilum-type mevalonate pathway | ADP + (R)-3-phosphomevalonate | - |
? | |
ATP + (R)-mevalonate | Picrophilus torridus DSM 9790 | key enzyme of the Thermoplasma acidophilum-type mevalonate pathway | ADP + (R)-3-phosphomevalonate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Picrophilus torridus | Q6KZB1 | - |
- |
Picrophilus torridus DSM 9790 | Q6KZB1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Picrophilus torridus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-mevalonate | - |
Picrophilus torridus | ADP + (R)-3-phosphomevalonate | - |
? | |
ATP + (R)-mevalonate | key enzyme of the Thermoplasma acidophilum-type mevalonate pathway | Picrophilus torridus | ADP + (R)-3-phosphomevalonate | - |
? | |
ATP + (R)-mevalonate | - |
Picrophilus torridus DSM 9790 | ADP + (R)-3-phosphomevalonate | - |
? | |
ATP + (R)-mevalonate | key enzyme of the Thermoplasma acidophilum-type mevalonate pathway | Picrophilus torridus DSM 9790 | ADP + (R)-3-phosphomevalonate | - |
? | |
ATP + 3-hydroxyisovalerate | the potential of the enzyme in isobutene formation is due to the conversion of 3-hydroxyisovalerate to an unstable 3-phosphate intermediate that undergoes consequent spontaneous decarboxylation to form isobutene | Picrophilus torridus | ADP + phosphate + isobutene + CO2 | - |
? | |
ATP + 3-hydroxyisovalerate | the potential of the enzyme in isobutene formation is due to the conversion of 3-hydroxyisovalerate to an unstable 3-phosphate intermediate that undergoes consequent spontaneous decarboxylation to form isobutene | Picrophilus torridus DSM 9790 | ADP + phosphate + isobutene + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
M3K | - |
Picrophilus torridus |
PTO1356 | locus name | Picrophilus torridus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
(R)-mevalonate | pH 7.5, 30°C | Picrophilus torridus |
General Information | Comment | Organism |
---|---|---|
metabolism | key enzyme of the Thermoplasma acidophilum-type mevalonate pathway | Picrophilus torridus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
(R)-mevalonate | pH 7.5, 30°C | Picrophilus torridus |