Cloned (Comment) | Organism |
---|---|
wild-type and mutants | Cereibacter sphaeroides |
Protein Variants | Comment | Organism |
---|---|---|
R168Q | 300fold decrease in catalytic efficiency, 50fold increased Km for D-ribulose 5-phosphate | Cereibacter sphaeroides |
R173Q | 100fold increased Km for D-ribulose 5-phosphate | Cereibacter sphaeroides |
R186Q | decreased Km for D-ribulose 5-phosphate | Cereibacter sphaeroides |
R187Q | increased Km for D-ribulose 5-phosphate | Cereibacter sphaeroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
D-ribulose 5-phosphate | R186Q mutant, pH 8, 30°C | Cereibacter sphaeroides | |
0.096 | - |
D-ribulose 5-phosphate | wild-type enzyme, pH 8, 30°C | Cereibacter sphaeroides | |
0.55 | - |
D-ribulose 5-phosphate | R187Q mutant, pH 8, 30°C | Cereibacter sphaeroides | |
4.6 | - |
D-ribulose 5-phosphate | R168Q mutant, pH 8, 30°C | Cereibacter sphaeroides | |
10.5 | - |
D-ribulose 5-phosphate | R173Q mutant, pH 8, 30°C | Cereibacter sphaeroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate + ATP | - |
Cereibacter sphaeroides | D-ribulose 1,5-diphosphate + ADP | - |
? | |
D-ribulose 5-phosphate + trinitrophenyl-ATP | - |
Cereibacter sphaeroides | D-ribulose 1,5-diphosphate + trinitrophenyl-ADP | - |
? |