Cloned (Comment) | Organism |
---|---|
- |
Enterococcus gallinarum |
Crystallization (Comment) | Organism |
---|---|
to 2.2 A resolution. Access to the ATP-binding template is blocked by a bulky tyrosine residue. Substitution of this tyrosine by a smaller amino acid opens access to the ATP template | Enterococcus gallinarum |
Protein Variants | Comment | Organism |
---|---|---|
Y92A | residue Y92 blocks access to the ATP-binding template. Mutant shows 8fold decrease in km value for ATP | Enterococcus gallinarum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
GTP | wild-type, pH not specified in the publication, temperature not specified in the publication | Enterococcus gallinarum | |
0.094 | - |
GTP | mutant Y92A, pH not specified in the publication, temperature not specified in the publication | Enterococcus gallinarum | |
0.22 | - |
ATP | mutant Y92A, pH not specified in the publication, temperature not specified in the publication | Enterococcus gallinarum | |
1.6 | - |
ATP | wild-type, pH not specified in the publication, temperature not specified in the publication | Enterococcus gallinarum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus gallinarum | P96762 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + kanamycin | - |
Enterococcus gallinarum | ADP + kanamycin 2''-phosphate | - |
? | |
GTP + kanamycin | - |
Enterococcus gallinarum | GDP + kanamycin 2''-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
gentamicin resistance protein | - |
Enterococcus gallinarum |