Literature summary for 2.7.1.197 extracted from

Lolkema, J.S.; Kuiper, H.; ten Hoeve-Duurkens, R.H.; Robillard, G.T.
Mannitol-specific enzyme II of the phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli: Physical size of enzyme IImtl and its domains IIBA and IIC in the active state (1993), Biochemistry, 32, 1396-1400.

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Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Escherichia coli	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38000	-	1 * 38000 + x * 175000, domains IIBA and IIC, SDS-PAGE	Escherichia coli
175000	-	1 * 38000 + x * 175000, domains IIBA and IIC, SDS-PAGE	Escherichia coli
315000	-	globular protein solubilized in decylPEG detergent, gel filtration	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
[protein]-Npi-phospho-L-histidine + D-mannitol[side 1]	Escherichia coli	-	[protein]-L-histidine + D-mannitol 1-phosphate[side 2]	-	ir
[protein]-Npi-phospho-L-histidine + D-mannitol[side 1]	Escherichia coli ML308-225	-	[protein]-L-histidine + D-mannitol 1-phosphate[side 2]	-	ir

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Escherichia coli ML308-225	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
hexylagarose column chromatography and TSK-250 gel filtration	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
[protein]-Npi-phospho-L-histidine + D-mannitol[side 1]	-	Escherichia coli	[protein]-L-histidine + D-mannitol 1-phosphate[side 2]	-	ir
[protein]-Npi-phospho-L-histidine + D-mannitol[side 1]	-	Escherichia coli ML308-225	[protein]-L-histidine + D-mannitol 1-phosphate[side 2]	-	ir

Subunits

Subunits	Comment	Organism
?	1 * 38000 + x * 175000, domains IIBA and IIC, SDS-PAGE	Escherichia coli

Synonyms

Synonyms	Comment	Organism
mannitol-specific enzyme II	-	Escherichia coli
mtlI	-	Escherichia coli

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Release 2023.1 (January 2023)