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Literature summary for 2.7.1.197 extracted from
- Structural and functional domains of the mannitol-specific enzyme II of the E. coli phosphoenolpyruvate-dependent phosphotransferase system (1989), FEMS Microbiol. Rev., 63, 25-34.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain LGS322 | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-ethylmaleimide | - |
Escherichia coli |  |
| Trypsin | the enzyme is very sensitive to trypsin which cleaves it into 2 fragments (ca. 30 kDa each) with concomitant inactivation | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Escherichia coli | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
1 * 34000 + 1 * 29000, SDS-PAGE | Escherichia coli |
| 34000 | - |
1 * 34000 + 1 * 29000, SDS-PAGE | Escherichia coli |
| 60000 | 65000 | gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [protein]-Npi-phospho-L-histidine + D-mannitol[side 1] | Escherichia coli | - |
[protein]-L-histidine + D-mannitol 1-phosphate[side 2] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | - |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | substrate specificity: mannitol>>2-amino-2-deoxymannitol, 2-deoxymannitol, glucitol >> arabinitol, mannonic acid | Escherichia coli | ? | - |
? | |
| [protein]-Npi-phospho-L-histidine + D-mannitol[side 1] | - |
Escherichia coli | [protein]-L-histidine + D-mannitol 1-phosphate[side 2] | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | 1 * 34000 + 1 * 29000, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EIIMtl | - |
Escherichia coli |
| mannitol permease | - |
Escherichia coli |
| mannitol-specific enzyme II | - |
Escherichia coli |
| mtlA | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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