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Literature summary for 2.7.1.2 extracted from
- The purification and characterization of glucokinase from the thermophile Bacillus stearothermophilus (1986), Biochem. J., 237, 415-420.
General Stability
| General Stability | Organism |
|---|---|
| DTT stabilizes at 70°C | Geobacillus stearothermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Geobacillus stearothermophilus |  |
| AgNO3 | - |
Geobacillus stearothermophilus | |
| D-glucose 6-phosphate | - |
Geobacillus stearothermophilus | |
| D-maltose | - |
Geobacillus stearothermophilus | |
| HgCl2 | - |
Geobacillus stearothermophilus | |
| iodoacetamide | - |
Geobacillus stearothermophilus | |
| N-acetyl-alpha-D-glucosamine | - |
Geobacillus stearothermophilus | |
| N-ethylmaleimide | - |
Geobacillus stearothermophilus | |
| p-chlormercuribenzoate | - |
Geobacillus stearothermophilus | |
| p-hydroxymercuribenzoate | - |
Geobacillus stearothermophilus | |
| Pb(NO3)2 | - |
Geobacillus stearothermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
alpha-D-glucose | 30°C, pH 7.6 | Geobacillus stearothermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | no activation | Geobacillus stearothermophilus | |
| Co2+ | 68% of the activity with Mg2+ | Geobacillus stearothermophilus | |
| Mg2+ | required | Geobacillus stearothermophilus | |
| Mn2+ | 45% of the activity with Mg2+ | Geobacillus stearothermophilus | |
| Zn2+ | no activation | Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 87000 | - |
HPLC gel filtration | Geobacillus stearothermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glucose | Geobacillus stearothermophilus | - |
ADP + D-glucose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| large scale | Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 334 | - |
- |
Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + alpha-D-glucose | - |
Geobacillus stearothermophilus | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-glucose | - |
Geobacillus stearothermophilus | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-glucose | highly specific for D-glucose | Geobacillus stearothermophilus | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + N-acetyl-alpha-D-glucosamine | at pH 7.6: 50% of the activity with alpha-D-glucose, at pH 9.0: 5% of the activity with alpha-D-glucose | Geobacillus stearothermophilus | ADP + N-acetyl-alpha-D-glucosamine 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | alpha2, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Geobacillus stearothermophilus |
| 60 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
DTT stabilizes at 70°C | Geobacillus stearothermophilus |
| 70 | - |
complete loss of activity in 70 min in presence of DTT | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Geobacillus stearothermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10.5 | pH 6.0: about 30% of activity maximum, pH 10.5: about 80% of activity maximum | Geobacillus stearothermophilus |
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