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Literature summary for 2.7.1.20 extracted from

  • Lindberg, B.; Klenow, H.; Hansen, K.
    Some properties of partially purified mammalian adenosine kinase (1967), J. Biol. Chem., 242, 350-356.
    View publication on PubMed
Search for more literature for 2.7.1.20

Activating Compound

Activating Compound Comment Organism Structure
ATP 0.7 mM with 0.5 mM Mg2+, 0.9 mM with 1 mM Mg2+ and 1.5 mM with 2 mM Mg2+ Oryctolagus cuniculus

General Stability

General Stability Organism
GSH, 2-mercaptoethanol, Mg2+ and adenosine do not stabilize Oryctolagus cuniculus
rapid inactivation in aqueous solution Oryctolagus cuniculus
unstable upon ammonium sulfate fractionation or adsorption on aluminium oxide or calcium phosphate gel Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
6-mercaptopurine riboside no inhibition Oryctolagus cuniculus
additional information
-
 Mus musculus
additional information inosine, aminonucleoside of puromycin, 2'-deoxyadenosine N1-oxide, 3'-deoxyadenosine N1-oxide, 8-aza-2'-deoxyadenosine, 2-aminopurine-2'-deoxyriboside, thioguanosine, psicofuranine, N6-methyl-2'-deoxyadenosine, N1-methyladenosine do not inhibit the enzyme activity Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 arabinofuranosyladenine pH 5.8, 25ºC Oryctolagus cuniculus
0.14
-
 4'-thioadenosine pH 5.8, 25ºC Oryctolagus cuniculus
0.15
-
 xylofuranosyladenine pH 5.8, 25ºC Oryctolagus cuniculus
0.16
-
 adenosine pH 5.8, 25ºC Oryctolagus cuniculus
0.16
-
 N6-methyladenosine pH 5.8, 25ºC Oryctolagus cuniculus
0.18
-
 2'-deoxyadenosine pH 5.8, 25ºC Oryctolagus cuniculus
0.34
-
 2'-amino-2',3'-dideoxyadenosine pH 5.8, 25ºC Oryctolagus cuniculus
0.35
-
 3'-deoxyadenosine pH 5.8, 25ºC, natural Oryctolagus cuniculus
0.4
-
 tubercidin pH 5.8, 25ºC Oryctolagus cuniculus
0.41
-
 adenosine-N1-oxide pH 5.8, 25ºC Oryctolagus cuniculus
0.42
-
 2,6-diaminopurine riboside pH 5.8, 25ºC Oryctolagus cuniculus
0.43
-
 2-fluoroadenosine pH 5.8, 25ºC Oryctolagus cuniculus
0.46
-
 3'-deoxyadenosine pH 5.8, 25ºC, natural Mus musculus
0.47
-
 3'-deoxyadenosine pH 5.8, 25ºC, synthetic Oryctolagus cuniculus
0.54
-
 N6-methyl-3'-deoxyadenosine pH 5.8, 25ºC Oryctolagus cuniculus
0.55
-
 6-Chloropurine riboside pH 5.8, 25ºC Oryctolagus cuniculus
0.58
-
 adenosine pH 5.8, 25ºC Mus musculus
0.61
-
 3'-amino-3'-deoxyadenosine pH 5.8, 25ºC Oryctolagus cuniculus
0.78
-
 purine riboside pH 5.8, 25ºC Oryctolagus cuniculus
0.8
-
 Toyocamycin pH 5.8, 25ºC Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Mus musculus 5829
-
cytosol
-
 Oryctolagus cuniculus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Mus musculus
Mg2+ requirement Oryctolagus cuniculus
Mg2+ optimal at Mg: ATP ratio 1 Mus musculus
Mg2+ optimal at Mg: ATP ratio 1 Oryctolagus cuniculus
Mg2+ 0.5 mM Mus musculus
Mg2+ 0.5 mM Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 gel filtration Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-
Oryctolagus cuniculus
-
 rabbit
-

Purification (Commentary)

Purification (Comment) Organism
chromatography on Sephadex G-100 Mus musculus
chromatography on Sephadex G-100 Oryctolagus cuniculus
partial Mus musculus
partial Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
Ehrlich ascites carcinoma cell
-
 Mus musculus
-
liver
-
 Mus musculus
-
liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.06 0.1
-
 Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
-18°C, in 20% glycerol, at least 6 months Mus musculus
-18°C, in 20% glycerol, at least 6 months Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'-amino-2',3'-dideoxyadenosine
-
 Oryctolagus cuniculus ADP + 2'-amino-2',3'-dideoxyadenosine 5'-phosphate
-
 ?
ATP + 2'-deoxy-adenosine
-
 Oryctolagus cuniculus ADP + 2'-deoxy-AMP
-
 ?
ATP + 2,6-diaminopurine riboside
-
 Oryctolagus cuniculus ADP + 2,6-diaminopurine riboside 5'-phosphate
-
 ?
ATP + 2-fluoroadenosine
-
 Oryctolagus cuniculus ADP + 2-fluoroadenosine 5'-phosphate
-
 ?
ATP + 3'-amino-3'-deoxyadenosine
-
 Oryctolagus cuniculus ADP + 3'-amino-3'-deoxyadenosine 5'-phosphate
-
 ?
ATP + 4'-thioadenosine
-
 Oryctolagus cuniculus ADP + 4'-thioadenosine 5'-phosphate
-
 ?
ATP + 6-chloropurine riboside
-
 Oryctolagus cuniculus ADP + 6-chloropurine riboside 5'-phosphate
-
 ?
ATP + 9-[beta-D-ribofuranosyl]-purine
-
 Oryctolagus cuniculus ADP + 9-[beta-D-ribofuranosyl]-purine 5'-phosphate
-
 ?
ATP + adenosine MgATP2- is the true substrate Mus musculus ADP + AMP
-
 ?
ATP + adenosine MgATP2- is the true substrate Oryctolagus cuniculus ADP + AMP
-
 ?
ATP + adenosine-1-N-oxide
-
 Oryctolagus cuniculus ADP + adenosine-1-N-oxide 5'-phosphate
-
 ?
ATP + arabinofuranosyladenine
-
 Oryctolagus cuniculus ADP + arabinofuranosyladenine 5'-phosphate
-
 ?
ATP + cordycepin
-
 Mus musculus ADP + cordycepin 5'-phosphate
-
 ?
ATP + cordycepin
-
 Oryctolagus cuniculus ADP + cordycepin 5'-phosphate
-
 ?
ATP + N6-methyl-3'-deoxyadenosine
-
 Oryctolagus cuniculus ADP + N6-methyl-3'-deoxyadenosine 5'-phosphate
-
 ?
ATP + N6-methyladenosine
-
 Oryctolagus cuniculus ADP + N6-methyladenosine 5'-phosphate
-
 ?
ATP + toyocamycin
-
 Oryctolagus cuniculus ADP + toyocamycin 5'-phosphate
-
 ?
ATP + tubercidin
-
 Oryctolagus cuniculus ADP + tubercidin 5'-phosphate
-
 ?
ATP + xylofuranosyladenine
-
 Oryctolagus cuniculus ADP + xylofuranosyladenine 5'-phosphate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
-
 Mus musculus
37
-
 t1/2: 45 min, at pH 7.4 Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
 in Tris-maleate or phosphate buffer Oryctolagus cuniculus
6.5
-
 citrate-phosphate buffer Oryctolagus cuniculus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.4
-
 t1/2: 45 min at 37°C Mus musculus
7.4
-
 t1/2: 45 min at 37°C Oryctolagus cuniculus