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Literature summary for 2.7.1.20 extracted from
- Purification and properties of human placental adenosine kinase (1979), J. Biol. Chem., 254, 11388-11393.
General Stability
| General Stability | Organism |
|---|---|
| bovine serum albumin stabilizes | Homo sapiens |
| very unstable in dilute solution. Addition of albumin to reaction mixture in dilute enzyme preparations increases activity. MgATP2-, 1 mM, adenosine 5 mM, and 20% glycerol stabilize on a short term basis at 4ºC. Repeated freeze-thawing inactivates dilute enzyme solutions | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | strong inhibition above 1 mM | Homo sapiens |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | can partially substitute for Mg2+ | Homo sapiens | |
| Co2+ | activation | Homo sapiens | |
| Mg2+ | requirement | Homo sapiens | |
| Mn2+ | can partially substitute for Mg2+ | Homo sapiens | |
| Mn2+ | activation | Homo sapiens | |
| Ni2+ | can partially substitute for Mg2+ | Homo sapiens | |
| Ni2+ | activation | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37250 | - |
estimated from Stokes radius in polyacrylamide gel electrophoresis | Homo sapiens |
| 38250 | - |
sucrose density gradient centrifugation | Homo sapiens |
| 40740 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ion exchange and affinity chromatography, partial, 3600fold purification | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Homo sapiens | - |
| kidney | - |
Homo sapiens | - |
| liver | - |
Homo sapiens | - |
| additional information | tissue distribution | Homo sapiens | - |
| pancreas | - |
Homo sapiens | - |
| placenta | - |
Homo sapiens | - |
| spleen | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, 0.5 mg protein/ml or more, purified preparation, at least 8 months | Homo sapiens |
| 4°C, glycerol, dithiothreitol and adenosine stabilize. 20% glycerol, t1/2 20 days, 75% loss of activity within 60 days. Without glycerol, t1/2 3 days. -70°C to 4°C, 0.01 mg protein/ml, 75 to 95% loss of activity within days | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + adenosine | best substrates | Homo sapiens | ADP + AMP | - |
? | |
| GTP + adenosine | - |
Homo sapiens | GDP + AMP | - |
? | |
| ITP + adenosine | - |
Homo sapiens | IDP + AMP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 38800-47720, SDS-PAGE | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
crude placental supernatant | Homo sapiens |
| 5.5 | - |
purified preparation, under ATP and adenosine saturating conditions | Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
more stable than at pH 5.5 | Homo sapiens |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Homo sapiens | isoelectric focusing | - |
5.9 |
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