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Literature summary for 2.7.1.20 extracted from
- Purification and properties of adenosine kinase from rat liver: separation from deoxyadenosine kinase activity (1985), Z. Naturforsch. C, 40, 34-41.
General Stability
| General Stability | Organism |
|---|---|
| at 1 mg protein/ml stable upon repeated freeze-thawing | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenosine | 0.001 mM | Rattus norvegicus |  |
| tubercidin | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | pH and Mg/ATP ratio-dependent Km | Rattus norvegicus | |
| 0.00015 | 0.0005 | adenosine | - |
Rattus norvegicus | |
| 0.36 | 0.67 | 2'-deoxy-adenosine | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Rattus norvegicus | |
| Mg2+ | reactive species: MgATP2- | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
1 * 52000, SDS-PAGE | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 18 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -25°C, 1 mg protein/ml, several months | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2'-deoxy-adenosine | - |
Rattus norvegicus | ADP + 2'-deoxy-AMP | - |
? | |
| ATP + adenosine | - |
Rattus norvegicus | ADP + AMP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 52000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
the pH-optimum is a function of the adenosine concentration | Rattus norvegicus |
| 5.5 | - |
0.1 mM adenosine | Rattus norvegicus |
| 5.7 | - |
0.01 mM adenosine | Rattus norvegicus |
| 6 | 8 | 0.0015 mM adenosine | Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.1 | 6.1 | about half-maximal activity at pH 5.1 and pH 6.1, 0.1 mM adenosine | Rattus norvegicus |
| 5.2 | 6.7 | about half-maximal activity at pH 5.2 and pH 6.7, 0.01 mM adenosine | Rattus norvegicus |
| 5.3 | 9 | about half-maximal activity at pH 5.3 and about 90% of maximal activity at pH 9.0, 0.0015 mM adenosine | Rattus norvegicus |
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Release 2023.1 (January 2023)
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