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Literature summary for 2.7.1.20 extracted from
- Adenosine kinase from Trypanosoma cruzi (1982), Biochem. Biophys. Res. Commun., 107, 381-388.
General Stability
| General Stability | Organism |
|---|---|
| ATP or GTP stabilizes during gel filtration | Trypanosoma cruzi |
| does not withstand dialysis against Tris-dithioerythritol buffer, pH 8, with or without 1 mM ATP | Trypanosoma cruzi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-Amino-5-imidazolecarboxamide riboside | - |
Trypanosoma cruzi |  |
| 6-dimethylaminopurine riboside | - |
Trypanosoma cruzi | |
| 6-mercaptopurine riboside | weak inhibition | Trypanosoma cruzi | |
| 6-methylaminopurine riboside | - |
Trypanosoma cruzi | |
| 6-methylmercaptopurine riboside phosphate | - |
Trypanosoma cruzi | |
| 8-azaguanosine | weak inhibition | Trypanosoma cruzi | |
| adenosine | - |
Trypanosoma cruzi | |
| ATP | no inhibition | Trypanosoma cruzi | |
| deoxyadenosine | - |
Trypanosoma cruzi | |
| Deoxycoformycin | - |
Trypanosoma cruzi | |
| deoxyguanosine | - |
Trypanosoma cruzi | |
| guanosine | weak inhibition | Trypanosoma cruzi | |
| Mg2+ | not | Trypanosoma cruzi | |
| tubercidin | - |
Trypanosoma cruzi | |
| Xanthosine | weak inhibition | Trypanosoma cruzi | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Trypanosoma cruzi | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Trypanosoma cruzi | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma cruzi | - |
trypanosomatid flagellate | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C to -20°C, 6 months | Trypanosoma cruzi |
| 4°C, with DTT or 2-mercaptoethanol, about 90% loss of activity, ATP or GTP does not stabilize | Trypanosoma cruzi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + adenosine | - |
Trypanosoma cruzi | ADP + AMP | - |
? | |
| GTP + adenosine | equally effective as ATP | Trypanosoma cruzi | GDP + AMP | - |
? | |
| ITP + adenosine | less than with ATP | Trypanosoma cruzi | IDP + AMP | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
assay at | Trypanosoma cruzi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
heat labile | Trypanosoma cruzi |
| 55 | - |
1 min, inactivation | Trypanosoma cruzi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Trypanosoma cruzi |
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Release 2023.1 (January 2023)
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