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Literature summary for 2.7.1.20 extracted from

  • Datta, R.; Das, I.; Sen, B.; Chakraborty, A.; Adak, S.; Mandal, C.; Datta, A.K.
    Mutational analysis of the active-site residues crucial for catalytic activity of adenosine kinase from Leishmania donovani (2005), Biochem. J., 387, 591-600.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain M15 Leishmania donovani

Protein Variants

Protein Variants Comment Organism
D299A site-directed mutagenesis, inactive mutant, slightly reduced AMP binding ability compared to the wild-type enzyme Leishmania donovani
G62D site-directed mutagenesis, inactive mutant, no AMP binding ability Leishmania donovani
R131A site-directed mutagenesis, inactive mutant, reduced catalytic activity and highly reduced AMP binding ability compared to the wild-type enzyme Leishmania donovani
R69A site-directed mutagenesis, reduced reduced catalytic activity and AMP binding ability compared to the wild-type enzyme Leishmania donovani
R69K site-directed mutagenesis, reduced catalytic activity and slightly reduced AMP binding ability compared to the wild-type enzyme Leishmania donovani
R69K/R131A site-directed mutagenesis, highly reduced catalytic activity and AMP binding ability compared to the wild-type enzyme Leishmania donovani

Inhibitors

Inhibitors Comment Organism Structure
Phenylglyoxal inhibition levels of wild-type and mutant enzymes, overview Leishmania donovani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
adenosine pH 7.5, 30°C, recombinant mutant R69A Leishmania donovani
0.016
-
adenosine pH 7.5, 30°C, recombinant mutants R131A and R69K/R131A Leishmania donovani
0.017
-
adenosine pH 7.5, 30°C, recombinant mutant R69K Leishmania donovani
0.02
-
adenosine pH 7.5, 30°C, recombinant wild-type enzyme Leishmania donovani
0.042
-
ATP pH 7.5, 30°C, recombinant wild-type enzyme Leishmania donovani
0.068
-
ATP pH 7.5, 30°C, recombinant mutant R69A Leishmania donovani
0.071
-
ATP pH 7.5, 30°C, recombinant mutant R131A Leishmania donovani
0.278
-
ATP pH 7.5, 30°C, recombinant mutant R69K Leishmania donovani
1.282
-
ATP pH 7.5, 30°C, recombinant mutant R69K/R131A Leishmania donovani

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ at 1.5 mM MgCl2 Leishmania donovani

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + adenosine Leishmania donovani
-
ADP + AMP
-
?

Organism

Organism UniProt Comment Textmining
Leishmania donovani
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain M15 by nickel affinity chromatography Leishmania donovani

Reaction

Reaction Comment Organism Reaction ID
ATP + adenosine = ADP + AMP residues G62, D299, R69, and R131 are crucial for catalytic activity Leishmania donovani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine
-
Leishmania donovani ADP + AMP
-
?

Subunits

Subunits Comment Organism
More modeling of the three-dimensional structures of wild-type and mutant enzymes, overview Leishmania donovani

Synonyms

Synonyms Comment Organism
ADK
-
Leishmania donovani

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Leishmania donovani

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.006
-
adenosine pH 7.5, 30°C, recombinant mutant R69K/R131A Leishmania donovani
0.008
-
ATP pH 7.5, 30°C, recombinant mutant R69K/R131A Leishmania donovani
0.029
-
ATP pH 7.5, 30°C, recombinant mutant R69A Leishmania donovani
0.032
-
ATP pH 7.5, 30°C, recombinant mutant R69K Leishmania donovani
0.038
-
adenosine pH 7.5, 30°C, recombinant mutant R69A Leishmania donovani
0.043
-
adenosine pH 7.5, 30°C, recombinant mutant R69K Leishmania donovani
0.052
-
ATP pH 7.5, 30°C, recombinant mutant R131A Leishmania donovani
0.065
-
adenosine pH 7.5, 30°C, recombinant mutant R131A Leishmania donovani
1.2
-
ATP pH 7.5, 30°C, recombinant wild-type enzyme Leishmania donovani
1.4
-
adenosine pH 7.5, 30°C, recombinant wild-type enzyme Leishmania donovani

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Leishmania donovani