Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain M15 | Leishmania donovani |
Protein Variants | Comment | Organism |
---|---|---|
D299A | site-directed mutagenesis, inactive mutant, slightly reduced AMP binding ability compared to the wild-type enzyme | Leishmania donovani |
G62D | site-directed mutagenesis, inactive mutant, no AMP binding ability | Leishmania donovani |
R131A | site-directed mutagenesis, inactive mutant, reduced catalytic activity and highly reduced AMP binding ability compared to the wild-type enzyme | Leishmania donovani |
R69A | site-directed mutagenesis, reduced reduced catalytic activity and AMP binding ability compared to the wild-type enzyme | Leishmania donovani |
R69K | site-directed mutagenesis, reduced catalytic activity and slightly reduced AMP binding ability compared to the wild-type enzyme | Leishmania donovani |
R69K/R131A | site-directed mutagenesis, highly reduced catalytic activity and AMP binding ability compared to the wild-type enzyme | Leishmania donovani |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Phenylglyoxal | inhibition levels of wild-type and mutant enzymes, overview | Leishmania donovani |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
adenosine | pH 7.5, 30°C, recombinant mutant R69A | Leishmania donovani | |
0.016 | - |
adenosine | pH 7.5, 30°C, recombinant mutants R131A and R69K/R131A | Leishmania donovani | |
0.017 | - |
adenosine | pH 7.5, 30°C, recombinant mutant R69K | Leishmania donovani | |
0.02 | - |
adenosine | pH 7.5, 30°C, recombinant wild-type enzyme | Leishmania donovani | |
0.042 | - |
ATP | pH 7.5, 30°C, recombinant wild-type enzyme | Leishmania donovani | |
0.068 | - |
ATP | pH 7.5, 30°C, recombinant mutant R69A | Leishmania donovani | |
0.071 | - |
ATP | pH 7.5, 30°C, recombinant mutant R131A | Leishmania donovani | |
0.278 | - |
ATP | pH 7.5, 30°C, recombinant mutant R69K | Leishmania donovani | |
1.282 | - |
ATP | pH 7.5, 30°C, recombinant mutant R69K/R131A | Leishmania donovani |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | at 1.5 mM MgCl2 | Leishmania donovani |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine | Leishmania donovani | - |
ADP + AMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain M15 by nickel affinity chromatography | Leishmania donovani |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + adenosine = ADP + AMP | residues G62, D299, R69, and R131 are crucial for catalytic activity | Leishmania donovani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine | - |
Leishmania donovani | ADP + AMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | modeling of the three-dimensional structures of wild-type and mutant enzymes, overview | Leishmania donovani |
Synonyms | Comment | Organism |
---|---|---|
ADK | - |
Leishmania donovani |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Leishmania donovani |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
adenosine | pH 7.5, 30°C, recombinant mutant R69K/R131A | Leishmania donovani | |
0.008 | - |
ATP | pH 7.5, 30°C, recombinant mutant R69K/R131A | Leishmania donovani | |
0.029 | - |
ATP | pH 7.5, 30°C, recombinant mutant R69A | Leishmania donovani | |
0.032 | - |
ATP | pH 7.5, 30°C, recombinant mutant R69K | Leishmania donovani | |
0.038 | - |
adenosine | pH 7.5, 30°C, recombinant mutant R69A | Leishmania donovani | |
0.043 | - |
adenosine | pH 7.5, 30°C, recombinant mutant R69K | Leishmania donovani | |
0.052 | - |
ATP | pH 7.5, 30°C, recombinant mutant R131A | Leishmania donovani | |
0.065 | - |
adenosine | pH 7.5, 30°C, recombinant mutant R131A | Leishmania donovani | |
1.2 | - |
ATP | pH 7.5, 30°C, recombinant wild-type enzyme | Leishmania donovani | |
1.4 | - |
adenosine | pH 7.5, 30°C, recombinant wild-type enzyme | Leishmania donovani |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Leishmania donovani |