Any feedback?
Literature summary for 2.7.1.20 extracted from
- Molecular characterization of recombinant mouse adenosine kinase and evaluation as a target for protein phosphorylation (2004), Eur. J. Biochem., 271, 3547-3555.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| 2 isozymes AK-L and AK-S, DNA and amino acid sequence determination and analysis, overexpression of His-tagged proteins in Escherichia coli strain BL21(DE3) | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00002 | - |
adenosine | recombinant isozymes AK-S and AK-L, pH 7.5, 37°C | Mus musculus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43500 | - |
x * 45000, recombinant His-tagged isozyme AK-L, SDS-PAGE, x * 43500, recombinant His-tagged isozyme AK-S, SDS-PAGE | Mus musculus |
| 45000 | - |
x * 45000, recombinant His-tagged isozyme AK-L, SDS-PAGE, x * 43500, recombinant His-tagged isozyme AK-S, SDS-PAGE | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + adenosine | Mus musculus | - |
ADP + AMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | P55264 | isozymes AK-L and AK-S; a long and a short isozyme, i.e. AK-L and AK-S | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | the recombinant isozymes AK-L and AK-S became phosphorylated by protein kinase C, no significant phosphorylation level by protein kinases PKA, PKG, MAPK, CK2, and Cdk1, overview | Mus musculus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged isozymes AK-L and AK-S from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Mus musculus | - |
| brain stem | low expression level | Mus musculus | - |
| central nervous system | - |
Mus musculus | - |
| cerebellum | - |
Mus musculus | - |
| heart | low expression level | Mus musculus | - |
| hippocampus | - |
Mus musculus | - |
| hypothalamus | - |
Mus musculus | - |
| intestine | low expression level | Mus musculus | - |
| kidney | - |
Mus musculus | - |
| liver | highest enzyme expression level | Mus musculus | - |
| lung | low expression level | Mus musculus | - |
| medial septum | brain, low expression level | Mus musculus | - |
| midbrain | low expression level | Mus musculus | - |
| additional information | isozyme AK-L and AK-S expression patterns | Mus musculus | - |
| spleen | - |
Mus musculus | - |
| testis | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + adenosine | - |
Mus musculus | ADP + AMP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, recombinant His-tagged isozyme AK-L, SDS-PAGE, x * 43500, recombinant His-tagged isozyme AK-S, SDS-PAGE | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AK | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
inactivation at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Mus musculus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
