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Literature summary for 2.7.1.20 extracted from

  • Galazka, J.; Striepen, B.; Ullman, B.
    Adenosine kinase from Cryptosporidium parvum (2006), Mol. Biochem. Parasitol., 149, 223-230.
    View publication on PubMed
Search for more literature for 2.7.1.20

Cloned(Commentary)

Cloned (Comment) Organism
CpAK gene codon-optimized for expression in Escherichia coli Cryptosporidium parvum

Inhibitors

Inhibitors Comment Organism Structure
2-chloroadenosine adenosine phosphorylation is inhibited by 100fold excess of the inhibitor Cryptosporidium parvum
4-nitro-6-benzylthioguanosine 0.2 mM, partial inhibition Cryptosporidium parvum
4-nitro-6-benzylthioinosine 0.2 mM, partial inhibition Cryptosporidium parvum
5'-amino-5'-deoxyadenosine adenosine phosphorylation is inhibited by 100fold excess of the inhibitor Cryptosporidium parvum
5'-deoxyadenosine adenosine phosphorylation is inhibited by 100fold excess of the inhibitor Cryptosporidium parvum
adenosine strongly inhibited by 100fold excess adenosine Cryptosporidium parvum
EDTA 100 mM, complete inhibition Cryptosporidium parvum
tubercidin adenosine phosphorylation is inhibited by 100fold excess of the inhibitor Cryptosporidium parvum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
 adenosine 37°C, pH 8.0 Cryptosporidium parvum
0.041
-
 ATP 37°C, pH 8.0 Cryptosporidium parvum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activity is dependent on divalent cation. Activity is highest in presence of Mg2+ (5 mM) or Fe2+ (5 mM). 70% of maximal activation with Mn2+ (5 mM) or Ca2+ (5 mM). 35% of maximal activation with Co2+ (5 mM) or Zn2+ (5 mM) Cryptosporidium parvum
Co2+ activity is dependent on divalent cation. Activity is highest in presence of Mg2+ (5 mM) or Fe2+ (5 mM). 70% of maximal activation with Mn2+ (5 mM) or Ca2+ (5 mM). 35% of maximal activation with Co2+ (5 mM) or Zn2+ (5 mM) Cryptosporidium parvum
Fe2+ activity is dependent on divalent cation. Activity is highest in presence of Mg2+ (5 mM) or Fe2+ (5 mM). 70% of maximal activation with Mn2+ (5 mM) or Ca2+ (5 mM). 35% of maximal activation with Co2+ (5 mM) or Zn2+ (5 mM) Cryptosporidium parvum
Mg2+ activity is dependent on divalent cation. Activity is highest in presence of Mg2+ (5 mM) or Fe2+ (5 mM). 70% of maximal activation with Mn2+ (5 mM) or Ca2+ (5 mM). 35% of maximal activation with Co2+ (5 mM) or Zn2+ (5 mM) Cryptosporidium parvum
Mn2+ activity is dependent on divalent cation. Activity is highest in presence of Mg2+ (5 mM) or Fe2+ (5 mM). 70% of maximal activation with Mn2+ (5 mM) or Ca2+ (5 mM). 35% of maximal activation with Co2+ (5 mM) or Zn2+ (5 mM) Cryptosporidium parvum
Zn2+ activity is dependent on divalent cation. Activity is highest in presence of Mg2+ (5 mM) or Fe2+ (5 mM). 70% of maximal activation with Mn2+ (5 mM) or Ca2+ (5 mM). 35% of maximal activation with Co2+ (5 mM) or Zn2+ (5 mM) Cryptosporidium parvum

Organism

Organism UniProt Comment Textmining
Cryptosporidium parvum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Cryptosporidium parvum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine no phosphorylation of inosine or guanosine Cryptosporidium parvum ADP + AMP
-
 ?
CTP + adenosine about 60% of activity with ATP Cryptosporidium parvum CDP + AMP
-
 ?
GTP + adenosine about 50% of activity with ATP Cryptosporidium parvum GDP + AMP
-
 ?
TTP + adenosine about 10% of activity with ATP Cryptosporidium parvum TDP + AMP
-
 ?
UTP + adenosine about 40% of activity with ATP Cryptosporidium parvum UDP + AMP
-
 ?

Synonyms

Synonyms Comment Organism
CpAK
-
 Cryptosporidium parvum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6
-
 ATP 37°C, pH 8.0 Cryptosporidium parvum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Cryptosporidium parvum

pH Range

pH Minimum pH Maximum Comment Organism
5.5 10.5 pH 5.5: about 40% of maximal activity, pH 10.5: about 65% of maximal activity Cryptosporidium parvum