Literature summary for 2.7.1.20 extracted from

Kuettel, S.; Greenwald, J.; Kostrewa, D.; Ahmed, S.; Scapozza, L.; Perozzo, R.
Crystal structures of T. b. rhodesiense adenosine kinase complexed with inhibitor and activator: implications for catalysis and hyperactivation (2011), PLoS Negl. Trop. Dis., 5, e1164.

Activating Compound

Activating Compound	Comment	Organism
3-(4-ethylphenyl)-5-(tetrahydro-2H-pyran-4-yl)-1H-pyrazole	-	Trypanosoma brucei
3-(4-phenoxyphenyl)-5-(tetrahydro-2H-pyran-4-yl)-1H-pyrazole	-	Trypanosoma brucei
4-[3-(4-chlorophenyl)-1H-pyrazol-5-yl]morpholine	-	Trypanosoma brucei
4-[5-(4-phenoxyphenyl)-2H-pyrazol-3-yl]morpholine	best activator	Trypanosoma brucei
4-[[3-(4-phenoxyphenyl)-1H-pyrazol-5-yl]methyl]morpholine	-	Trypanosoma brucei

Cloned(Commentary)

Cloned (Comment)	Organism
-	Trypanosoma brucei

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with the bisubstrate inhibitor P1,P5-di(adenosine-5')-pentaphosphate (at 16°C, in 0.2 M sodium acetate trihydrate, 0.1 M sodium cacodylate, pH 6.0, and 24% (w/v) polyethylene glycol 8000 (crystal form 1), or in 0.1 M tri-sodium citrate dihydrate, pH 5.6, 20% (v/v) isopropanol, and 20% (w/v) polytethylene glycol 4000 (crystal form 2)) and with the activator 4-[5-(4-phenoxyphenyl)-2H-pyrazol-3-yl]morpholine (at 4°C in 0.1 M Tris, pH 9.0 and 60% (v/v) 2-methyl-2,4-pentanediol), sitting drop vapor diffusion method	Trypanosoma brucei

Crystallization (Comment)

Organism

in complex with the bisubstrate inhibitor P1,P5-di(adenosine-5')-pentaphosphate (at 16°C, in 0.2 M sodium acetate trihydrate, 0.1 M sodium cacodylate, pH 6.0, and 24% (w/v) polyethylene glycol 8000 (crystal form 1), or in 0.1 M tri-sodium citrate dihydrate, pH 5.6, 20% (v/v) isopropanol, and 20% (w/v) polytethylene glycol 4000 (crystal form 2)) and with the activator 4-[5-(4-phenoxyphenyl)-2H-pyrazol-3-yl]morpholine (at 4°C in 0.1 M Tris, pH 9.0 and 60% (v/v) 2-methyl-2,4-pentanediol), sitting drop vapor diffusion method

Trypanosoma brucei

Inhibitors

Inhibitors	Comment	Organism	Structure
P1,P5-di(adenosine-5')pentaphosphate	-	Trypanosoma brucei

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Trypanosoma brucei

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma brucei	Q584S0	subsp. rhodesiense	-

Purification (Commentary)

Purification (Comment)	Organism
-	Trypanosoma brucei

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + adenosine	ATP is the preferred phosphoryl donor	Trypanosoma brucei	ADP + AMP	-	?

Synonyms

Synonyms	Comment	Organism
ATP:adenosine 5'-phosphotransferase	-	Trypanosoma brucei

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
43	-	the melting temperature of the apo-enzyme is at 43°C	Trypanosoma brucei

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0294	-	pH and temperature not specified in the publication	Trypanosoma brucei	P1,P5-di(adenosine-5')-pentaphosphate