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Literature summary for 2.7.1.207 extracted from

  • Gunnewijk, M.; Postma, P.; Poolman, B.
    Phosphorylation and functional properties of the IIA domain of the lactose transport protein of Streptococcus thermophilus (1999), J. Bacteriol., 181, 632-641.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.207

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Streptococcus thermophilus

Protein Variants

Protein Variants Comment Organism
I548E/G556D the mutant of domain IIA has increased phosphoryl transfer activity Streptococcus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 x * 20000, His-tagged domain IIALacS, SDS-PAGE Streptococcus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[protein]-Npi-phospho-L-histidine + lactose[side 1] Streptococcus thermophilus
-
 [protein]-L-histidine + lactose 6'-phosphate[side 2]
-
 ?
[protein]-Npi-phospho-L-histidine + lactose[side 1] Streptococcus thermophilus ST11
-
 [protein]-L-histidine + lactose 6'-phosphate[side 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus thermophilus
-
-
-
Streptococcus thermophilus ST11
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein domain IIALacS is phosphorylated, most likely at His-552 Streptococcus thermophilus

Purification (Commentary)

Purification (Comment) Organism
S-Sepharose column chromatography and DEAE-Sepharose column chromatography Streptococcus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[protein]-Npi-phospho-L-histidine + lactose[side 1]
-
 Streptococcus thermophilus [protein]-L-histidine + lactose 6'-phosphate[side 2]
-
 ?
[protein]-Npi-phospho-L-histidine + lactose[side 1]
-
 Streptococcus thermophilus ST11 [protein]-L-histidine + lactose 6'-phosphate[side 2]
-
 ?

Subunits

Subunits Comment Organism
? x * 20000, His-tagged domain IIALacS, SDS-PAGE Streptococcus thermophilus

Synonyms

Synonyms Comment Organism
IIALacS regulatory domain Streptococcus thermophilus
lactose transport protein
-
 Streptococcus thermophilus