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Literature summary for 2.7.1.21 extracted from
- Purification and characterization of thymidine kinase from regenerating rat liver (1991), Biochim. Biophys. Acta, 1079, 348-352.
General Stability
| General Stability | Organism |
|---|---|
| dithiothreitol, 5 mM stabilizes | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | competitive to ATP | Rattus norvegicus |  |
| dTMP | competitive to thymidine | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0022 | - |
thymidine | pH 8, 37°C | Rattus norvegicus | |
| 0.2 | - |
ATP | pH 8, 37°C | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
2 * 26000, SDS-PAGE | Rattus norvegicus |
| 54000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| homogeneity | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + thymidine | ATP can be substituted by GTP | Rattus norvegicus | ADP + dTMP | - |
? | |
| ATP + thymidine | ATP can be substituted by dGTP | Rattus norvegicus | ADP + dTMP | - |
? | |
| ATP + thymidine | ATP can be substituted by UTP | Rattus norvegicus | ADP + dTMP | - |
? | |
| ATP + thymidine | ATP can be substituted by dATP | Rattus norvegicus | ADP + dTMP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 26000, SDS-PAGE | Rattus norvegicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
ADP | with ATP as variable substrate, pH 8, 37°C | Rattus norvegicus | |
| 0.65 | - |
dTMP | with ATP or thymidine as variable substrate, pH 8, 37°C | Rattus norvegicus | |
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Release 2023.1 (January 2023)
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