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Literature summary for 2.7.1.21 extracted from

  • Solaroli, N.; Johansson, M.; Balzarini, J.; Karlsson, A.
    Substrate specificity of three viral thymidine kinases (TK): vaccinia virus TK, feline herpesvirus TK, and canine herpesvirus TK (2006), Nucleosides Nucleotides Nucleic Acids, 25, 1189-1192.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as poly-histidine fusion protein in the pET-16b vector Vaccinia virus
expression in Escherichia coli as poly-histidine fusion protein in the pET-16b vector Felid alphaherpesvirus 1
expression in Escherichia coli as poly-histidine fusion protein in the pET-16b vector Canid alphaherpesvirus 1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
3'-azido-3'-deoxythymidine 37°C, pH 7.6 Felid alphaherpesvirus 1
0.016
-
2'-deoxythymidine 37°C, pH 7.6 Canid alphaherpesvirus 1
0.041
-
2'-deoxythymidine 37°C, pH 7.6 Felid alphaherpesvirus 1
0.05
-
3'-azido-3'-deoxythymidine 37°C, pH 7.6 Canid alphaherpesvirus 1
0.33
-
2'-deoxythymidine 37°C, pH 7.6 Vaccinia virus
0.6
-
3'-azido-3'-deoxythymidine 37°C, pH 7.6 Vaccinia virus

Organism

Organism UniProt Comment Textmining
Canid alphaherpesvirus 1
-
-
-
Felid alphaherpesvirus 1
-
-
-
Vaccinia virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Vaccinia virus
-
Felid alphaherpesvirus 1
-
Canid alphaherpesvirus 1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine about 115% of the activity with 2'-deoxythymidine Canid alphaherpesvirus 1 ADP + (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate
-
?
ATP + 1-beta-D-arabinofuranosylthymine as active as with 2'-deoxythymidine Canid alphaherpesvirus 1 ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate
-
?
ATP + 2'-deoxythymidine
-
Vaccinia virus ADP + 2'-deoxythymidine 5'-phosphate
-
?
ATP + 2'-deoxythymidine
-
Felid alphaherpesvirus 1 ADP + 2'-deoxythymidine 5'-phosphate
-
?
ATP + 2'-deoxythymidine
-
Canid alphaherpesvirus 1 ADP + 2'-deoxythymidine 5'-phosphate
-
?
ATP + 3'-azido-2',3'-dideoxythymidine about 165% of the activity with 2'-deoxythymidine Vaccinia virus ADP + 3'-azido-2',3'-dideoxythymidine 5'-phosphate
-
?
ATP + 3'-azido-2',3'-dideoxythymidine about 5% of the activity with 2'-deoxythymidine Canid alphaherpesvirus 1 ADP + 3'-azido-2',3'-dideoxythymidine 5'-phosphate
-
?
ATP + 3'-azido-3'-deoxythymidine
-
Felid alphaherpesvirus 1 ADP + 3'-azido-3'-deoxythymidine 5'-phosphate
-
?

Synonyms

Synonyms Comment Organism
CHV-TK
-
Canid alphaherpesvirus 1
FHV-TK
-
Felid alphaherpesvirus 1
VVTK
-
Vaccinia virus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
2'-deoxythymidine 37°C, pH 7.6 Canid alphaherpesvirus 1
0.53
-
3'-azido-3'-deoxythymidine 37°C, pH 7.6 Canid alphaherpesvirus 1
0.97
-
3'-azido-3'-deoxythymidine 37°C, pH 7.6 Felid alphaherpesvirus 1
6.47
-
2'-deoxythymidine 37°C, pH 7.6 Canid alphaherpesvirus 1
23
-
2'-deoxythymidine 37°C, pH 7.6 Felid alphaherpesvirus 1
220
-
2'-deoxythymidine 37°C, pH 7.6 Vaccinia virus
412
-
3'-azido-3'-deoxythymidine 37°C, pH 7.6 Vaccinia virus