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Literature summary for 2.7.1.23 extracted from
- NAD+ kinase from sea urchin eggs (1980), Methods Enzymol., 66, 101-104.
General Stability
| General Stability | Organism |
|---|---|
| dialysis at 4°C for 18 h against buffer at pH 8.5 results in loss of 80% of the activity | Strongylocentrotus purpuratus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Strongylocentrotus purpuratus |  |
| NADPH | - |
Strongylocentrotus purpuratus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 5 mM, 33% of the activation obtained with Mn2+ | Strongylocentrotus purpuratus | |
| Mg2+ | divalent cation required | Strongylocentrotus purpuratus | |
| Mg2+ | Mg2+ at 1 mM gives 60% of the maximum activity obtained with Mn2+ | Strongylocentrotus purpuratus | |
| Mn2+ | divalent cation required | Strongylocentrotus purpuratus | |
| Mn2+ | maximal activity at 0.5 mM | Strongylocentrotus purpuratus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Strongylocentrotus purpuratus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Strongylocentrotus purpuratus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| egg | - |
Strongylocentrotus purpuratus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.093 | - |
- |
Strongylocentrotus purpuratus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stored as a slurry in 60% saturated (NH4)2SO4 stable for at least 2 weeks | Strongylocentrotus purpuratus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + NAD+ | - |
Strongylocentrotus purpuratus | ADP + NADP+ | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Strongylocentrotus purpuratus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | activity decreases sharply below pH 6.0 and above pH 7.0 | Strongylocentrotus purpuratus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7 | 4°C, stable for at least 48 h, irreversible loss of activity below pH 5.5 | Strongylocentrotus purpuratus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Strongylocentrotus purpuratus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
NADPH | - |
Strongylocentrotus purpuratus | |
| 0.03 | - |
NADH | - |
Strongylocentrotus purpuratus | |
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Release 2023.1 (January 2023)
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