General Stability | Organism |
---|---|
stability is enhanced by immobilization on CNBr-activated Sepharose 4B | Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
NAD+ | native enzyme | Gallus gallus | |
1.5 | - |
NAD+ | enzyme immobilized on CNBr-activated Sepharose 4B | Gallus gallus | |
2.5 | - |
ATP | native enzyme | Gallus gallus | |
4.5 | - |
ATP | enzyme immobilized on CNBr-activated Sepharose 4B | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Gallus gallus | - |
Storage Stability | Organism |
---|---|
4°C, 0.1 M phosphate buffer, pH 7.5, activity of the native enzyme is entirely lost after 55 days, enzyme immobilized and CNBr-activated Sepharose 4B retains activity without any detectable loss of after 70 days | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NAD+ | - |
Gallus gallus | ADP + NADP+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
native enzyme | Gallus gallus |
55 | - |
enzyme immobilized on CNBr-activated Sepharose 4B | Gallus gallus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme immobilized on CNBr-activated Sepharose is more stable against heat than the native enzyme | Gallus gallus |
40 | - |
native enzyme and immobilized enzyme, stable | Gallus gallus |
55 | - |
immobilized enzyme is more stable than the native enzyme above 55°C | Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
enzyme immobilized on CNBr-activated Sepharose 4B, phosphate buffer | Gallus gallus |
7 | - |
native enzyme, phosphate buffer, Tris-HCl buffer | Gallus gallus |
7.5 | - |
enzyme immobilized on CNBr-activated Sepharose 4B, Tris-HCl buffer | Gallus gallus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | 37°C, 2 h, native and immobilized stable | Gallus gallus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Gallus gallus |