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Literature summary for 2.7.1.23 extracted from

  • Zerez, C.R.; Moul, D.E.; Andreoli, A.J.
    NAD kinase from Bacillus licheniformis: inhibition by NADP and other properties (1986), Arch. Microbiol., 144, 313-316.
    View publication on PubMed
Search for more literature for 2.7.1.23

Inhibitors

Inhibitors Comment Organism Structure
NADP+ 0.1-0.2 mM, marked inhibition Bacillus licheniformis
PCMB 0.02 mM, 35% inhibition Bacillus licheniformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7
-
 NAD+ pH 6.8 Bacillus licheniformis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ several divalent cations satisfy the metal ion requirement: Mg2+, Mn2+, Ca2+, Fe2+, Zn2+ and Co2+. Most effective are Mn2+, Mg2+ and Ca2+. Maximal activity with Ca2+ at 9.0 mM Bacillus licheniformis
Ca2+ several divalent cations satisfy the metal ion requirement: Mg2+, Mn2+, Ca2+, Fe2+, Zn2+ and Co2+ Bacillus licheniformis
Co2+ several divalent cations satisfy the metal ion requirement: Mg2+, Mn2+, Ca2+, Fe2+, Zn2+ and Co2+ Bacillus licheniformis
Fe2+ several divalent cations satisfy the metal ion requirement: Mg2+, Mn2+, Ca2+, Fe2+, Zn2+ and Co2+ Bacillus licheniformis
Mg2+ several divalent cations satisfy the metal ion requirement: Mg2+, Mn2+, Ca2+, Fe2+, Zn2+ and Co2+. Most effective are Mn2+, Mg2+ and Ca2+. Maximal activity with Mg2+ at 7.5 mM Bacillus licheniformis
Mn2+ several divalent cations satisfy the metal ion requirement: Mg2+, Mn2+, Ca2+, Fe2+, Zn2+ and Co2+. Most effective are Mn2+, Mg2+ and Ca2+. Maximal activity with Mn2+ at 6.0 mM Bacillus licheniformis
Zn2+ several divalent cations satisfy the metal ion requirement: Mg2+, Mn2+, Ca2+, Fe2+, Zn2+ and Co2+ Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus licheniformis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.94
-
-
 Bacillus licheniformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + NAD+
-
 Bacillus licheniformis ADP + NADP+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Bacillus licheniformis

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7.2 90% of maximal activity at pH 6.5 and pH 7.2 Bacillus licheniformis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Bacillus licheniformis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.13
-
 NADP+ pH 6.8 Bacillus licheniformis