Literature summary for 2.7.1.23 extracted from

Oganesyan, V.; Huang, C.; Adams, P.D.; Jancarik, J.; Yokota, H.A.; Kim, R.; Kim, S.H.
Structure of a NAD kinase from Thermotoga maritima at 2.3 A resolution (2005), Acta Crystallogr. Sect. F, 61, 640-646.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals of SeMet-containing PPNK_THEMA are obtained at room temperature in hanging drops. Crystal structure of inorganic polyphosphate/ATP-NAD kinase is determined at 2.3 A resolution. The crystal structure is solved using single-wavelength anomalous diffraction data collected at the Se absorption-peak wavelength in a state in which no cofactors or substrates are bound. It revealed that the 258-amino-acid protein is folded into two distinct domains. The N-terminal alpha/beta-domain spans the first 100 amino acids and the last 30 amino acids of the polypeptide and has several topological matches in the PDB, whereas the other domain, which spans the middle 130 residues, adopts a unique beta-sandwich architecture and only appreciably matches the recently deposited PDB structures of NAD kinases	Thermotoga maritima

Crystallization (Comment)

Organism

crystals of SeMet-containing PPNK_THEMA are obtained at room temperature in hanging drops. Crystal structure of inorganic polyphosphate/ATP-NAD kinase is determined at 2.3 A resolution. The crystal structure is solved using single-wavelength anomalous diffraction data collected at the Se absorption-peak wavelength in a state in which no cofactors or substrates are bound. It revealed that the 258-amino-acid protein is folded into two distinct domains. The N-terminal alpha/beta-domain spans the first 100 amino acids and the last 30 amino acids of the polypeptide and has several topological matches in the PDB, whereas the other domain, which spans the middle 130 residues, adopts a unique beta-sandwich architecture and only appreciably matches the recently deposited PDB structures of NAD kinases

Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + NAD+	Thermotoga maritima	-	ADP + NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	Q9X255	-	-

Renatured (Commentary)

Renatured (Comment)	Organism
recombinant enzyme	Thermotoga maritima

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + NAD+	-	Thermotoga maritima	ADP + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
PPNK_THEMA	-	Thermotoga maritima