Activating Compound | Comment | Organism | Structure |
---|---|---|---|
5'-AMP | 1.56fold activation at 3.0 mM | Methanocaldococcus jannaschii |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | 3 mM, 29% inhibition | Methanocaldococcus jannaschii | |
NADP+ | 0.3 mM, complete inhibition | Methanocaldococcus jannaschii | |
NADPH | 0.3 mM, 18% inhibition | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
ATP | 85°C, pH 8.5, 20 mM Mg2+ | Methanocaldococcus jannaschii | |
3 | - |
NAD+ | 85°C, pH 8.5, 20 mM Mg2+ | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activity is dependent on Mg2+, maximal activity at 50 mM | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
99.4 | - |
- |
Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NAD+ | - |
Methanocaldococcus jannaschii | ADP + NADP+ | - |
? | |
ATP + NADH | 14% of the activity NAD+ | Methanocaldococcus jannaschii | ADP + NADPH | - |
? | |
CTP + NAD+ | 102% of the activity with ATP | Methanocaldococcus jannaschii | CDP + NADP+ | - |
? | |
dATP + NAD+ | 73% of the activity with ATP | Methanocaldococcus jannaschii | dADP + NADP+ | - |
? | |
GTP + NAD+ | 66% of the activity with ATP | Methanocaldococcus jannaschii | GDP + NADP+ | - |
? | |
hexametaphosphate + NAD+ | 47% of the activity with ATP | Methanocaldococcus jannaschii | NADP+ + ? | - |
? | |
additional information | the enzyme also shows phosphatase activity | Methanocaldococcus jannaschii | ? | - |
? | |
TTP + NAD+ | 71% of the activity with ATP | Methanocaldococcus jannaschii | TDP + NADP+ | - |
? | |
UTP + NAD+ | 114% of the activity with ATP | Methanocaldococcus jannaschii | UDP + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MJ0917 | - |
Methanocaldococcus jannaschii |
NADP phosphatase/NAD kinase | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
- |
Methanocaldococcus jannaschii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 100 | 80°C: about 45% of maximal activity, 100°C: optimum | Methanocaldococcus jannaschii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
399 | - |
ATP | 85°C, pH 8.5, 20 mM Mg2+ | Methanocaldococcus jannaschii | |
424 | - |
NAD+ | 85°C, pH 8.5, 20 mM Mg2+ | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Methanocaldococcus jannaschii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | pH 6.5: about 35% of maximal activity, pH 9.5: about 90% of maximal activity | Methanocaldococcus jannaschii |