Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-Phosphoadenosine 5'-phosphosulfate | - |
Penicillium chrysogenum | |
3'-phosphoadenosine 5'-phosphosulfate | product inhibition | Penicillium chrysogenum | |
adenosine 5'-phosphosulfate | kinetics | Penicillium chrysogenum | |
ATP | inhibition by free ATP, i.e. in excess of total Mg2+ | Penicillium chrysogenum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic study | Penicillium chrysogenum | |
0.0014 | - |
adenosine 5'-phosphosulfate | pH 8.0, 30°C | Penicillium chrysogenum | |
0.008 | - |
3'-phosphoadenosine 5'-phosphosulfate | pH 8.0, 30°C | Penicillium chrysogenum | |
1.5 | - |
ATP | pH 8.0, 30°C | Penicillium chrysogenum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 5 mM, 58% of activation with Mg2+ or Mn2+ | Penicillium chrysogenum | |
Mg2+ | activation | Penicillium chrysogenum | |
Mn2+ | 5 mM yield the same reaction velocity as 5 mM Mg2+, excess inhibits slightly | Penicillium chrysogenum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
2 * 30000, SDS-PAGE | Penicillium chrysogenum |
59000 | 60000 | gel filtration | Penicillium chrysogenum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine 5-phosphosulfate | Penicillium chrysogenum | second step in pathway of assimilation of inorganic sulfate | ADP + 3'-phosphoadenosine 5'-phosphosulfate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium chrysogenum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Affi-gel blue, Matrix-gel green, Agarose A | Penicillium chrysogenum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Penicillium chrysogenum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
24.7 | - |
- |
Penicillium chrysogenum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine 5-phosphosulfate | i.e. adenylylsulfate or APS | Penicillium chrysogenum | ADP + 3'-phosphoadenosine 5'-phosphosulfate | i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate | r | |
ATP + adenosine 5-phosphosulfate | second step in pathway of assimilation of inorganic sulfate | Penicillium chrysogenum | ADP + 3'-phosphoadenosine 5'-phosphosulfate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 30000, SDS-PAGE | Penicillium chrysogenum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Penicillium chrysogenum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
rapid loss of activity, approx. 80% of activity is recovered upon cooling at 0°C, presence of MgATP2- accelerates the recovery process | Penicillium chrysogenum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
90% of maximal activity at pH 7.5 and pH 8.5 | Penicillium chrysogenum |