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Literature summary for 2.7.1.25 extracted from

  • Satishchandran, C.; Hickman, Y.N.; Markham, G.D.
    Characterization of the phosphorylated enzyme intermediate formed in the adenosine 5-phosphosulfate kinase reaction (1992), Biochemistry, 31, 11684-11688.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
structural gene cysC Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22321
-
x * 22321, deduced from DNA sequence of cysC coding region Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + adenosine 5-phosphosulfate Escherichia coli ultimate step in biosynthesis of 3'-phosphoadenosine 5'-phosphosulfate, the primary biological sulfuryl donor ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification enzyme is phosphorylated upon incubation with ATP, phosphorylation site is identified as Ser109, phosphorylated enzyme is kinetically competent Escherichia coli

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate mechanism Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine 5-phosphosulfate in the absence of adenosine 5'-phosphosulfate ATP phosphorylates the enzyme at a rate equivalent to the overall kinase reaction, phosphorylation site: Ser-109 Escherichia coli ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?
ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Escherichia coli ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?
ATP + adenosine 5-phosphosulfate ultimate step in biosynthesis of 3'-phosphoadenosine 5'-phosphosulfate, the primary biological sulfuryl donor Escherichia coli ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
?

Subunits

Subunits Comment Organism
? x * 22321, deduced from DNA sequence of cysC coding region Escherichia coli