Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
in complex with adenosine 5'-phosphosulfate, hanging drop vapor diffusion method, using 100 mM HEPES, pH 7.25, 200 mM MgCl2, and 15–17.5% (w/v) PEG 2000 | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
D136A | the mutant displays drastically reduced affinity for adenosine 5'-phosphosulfate compared to the wild type enzyme | Arabidopsis thaliana |
D136N | the mutant displays drastically reduced affinity for adenosine 5'-phosphosulfate compared to the wild type enzyme | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine 5'-phosphosulfate | - |
Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00048 | - |
adenosine 5'-phosphosulfate | wild type enzyme, at pH 7.5 and 17°C | Arabidopsis thaliana | |
0.0007 | - |
adenosine 5'-phosphosulfate | mutant enzyme D136N, at pH 7.5 and 17°C | Arabidopsis thaliana | |
0.00092 | - |
adenosine 5'-phosphosulfate | mutant enzyme D136A, at pH 7.5 and 17°C | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q43295 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography and Superdex-200 gel filtration | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine 5'-phosphosulfate | - |
Arabidopsis thaliana | ADP + 3'-phosphoadenosine 5'-phosphosulfate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
adenosine 5Â’-phosphosulfate kinase | - |
Arabidopsis thaliana |
adenylyl-sulfate kinase | - |
Arabidopsis thaliana |
APS kinase | - |
Arabidopsis thaliana |
APSK | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
29 | - |
adenosine 5'-phosphosulfate | mutant enzyme D136A, at pH 7.5 and 17°C | Arabidopsis thaliana | |
45 | - |
adenosine 5'-phosphosulfate | mutant enzyme D136N, at pH 7.5 and 17°C | Arabidopsis thaliana | |
272 | - |
adenosine 5'-phosphosulfate | wild type enzyme, at pH 7.5 and 17°C | Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0328 | - |
adenosine 5'-phosphosulfate | mutant enzyme D136N, at pH 7.5 and 17°C | Arabidopsis thaliana | |
0.0375 | - |
adenosine 5'-phosphosulfate | wild type enzyme, at pH 7.5 and 17°C | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
31000 | - |
adenosine 5'-phosphosulfate | mutant enzyme D136A, at pH 7.5 and 17°C | Arabidopsis thaliana | |
64000 | - |
adenosine 5'-phosphosulfate | mutant enzyme D136N, at pH 7.5 and 17°C | Arabidopsis thaliana | |
567000 | - |
adenosine 5'-phosphosulfate | wild type enzyme, at pH 7.5 and 17°C | Arabidopsis thaliana |