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Literature summary for 2.7.1.26 extracted from
- Purification, properties, and function of flavokinase from rat intestinal mucosa (1990), J. Biochem., 107, 298-303.
General Stability
| General Stability | Organism |
|---|---|
| 50% of the activity is lost on freezing, but the stability of the enzyme is not greatly affected by the period of freezing | Rattus norvegicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 13500 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| intestinal mucosa | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.79 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 3°C, MOPS buffer, activity decreases to approximately 35, 30 and 13% of the initial level after 24, 48, and 96 h, respectively | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2'-deoxyriboflavin | 31% of the activity with riboflavin | Rattus norvegicus | ADP + 2'-deoxyriboflavin 5'-phosphate | - |
? |  |
| ATP + 8-bromo-8-demethylriboflavin | 384% of the activity with riboflavin | Rattus norvegicus | ADP + 8-bromo-8-demethylriboflavin 5-'phosphate | - |
? | |
| ATP + 8-chloro-8-demethylriboflavin | 122.2% of the activity with riboflavin | Rattus norvegicus | ADP + 8-chloro-8-demethylriboflavin 5'-phosphate | - |
? | |
| ATP + 8-demethylriboflavin | 110% of the activity with riboflavin | Rattus norvegicus | ADP + 8-demethylriboflavin 5'-phosphate | - |
? | |
| ATP + 8-dimethylamino-8-demethylriboflavin | 110% of the activity with riboflavin | Rattus norvegicus | ADP + 8-dimethylamino-8-demethylriboflavin 5'-phosphate | - |
? | |
| ATP + 8-ethoxy-8-demethylriboflavin | 210% of the activity with riboflavin | Rattus norvegicus | ADP + 8-ethoxy-8-demethylriboflavin 5'-phosphate | - |
? | |
| ATP + 8-fluoro-8-demethylriboflavin | 132.2% of the activity with riboflavin | Rattus norvegicus | ADP + 8-fluoro-8-demethylriboflavin 5'-phosphate | - |
? | |
| ATP + 8-iodo-8-demethylriboflavin | 334.7% of the activity with riboflavin | Rattus norvegicus | ADP + 8-iodo-8-demethylriboflavin 5'-phosphate | - |
? | |
| ATP + 8-methoxy-8-demethylriboflavin | 114.5% of the activity with riboflavin | Rattus norvegicus | ADP + 8-methoxy-8-demethylriboflavin 5'-phosphate | - |
? | |
| ATP + 8-methylamino-8-demethylriboflavin | 237.3% of the activity with riboflavin | Rattus norvegicus | ADP + 8-methylamino-8-demethylriboflavin 5'-phosphate | - |
? | |
| ATP + riboflavin | - |
Rattus norvegicus | ADP + FMN | - |
? | |
| ATP + roseoflavin | 187% of the activity with 8-demethylriboflavin | Rattus norvegicus | ADP + roseoflavin 5'-phosphate | - |
? | |
| ATP + roseoflavin | 81% of the activity with riboflavin | Rattus norvegicus | ADP + roseoflavin 5'-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 13500, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
enzyme from intestinal mucosa | Rattus norvegicus |
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