Any feedback?
Literature summary for 2.7.1.26 extracted from
- Continuous fluorescence assay, partial purification and properties of flavokinase from Megasphaera elsdenii (1980), Methods Enzymol., 66, 323-327.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0047 | - |
riboflavin | - |
Megasphaera elsdenii |  |
| 0.01 | - |
riboflavin | - |
Megasphaera elsdenii | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Megasphaera elsdenii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, affinity chromatography | Megasphaera elsdenii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0083 | - |
- |
Megasphaera elsdenii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + riboflavin | 22% of the activity with ATP | Megasphaera elsdenii | AMP + FMN | - |
? | |
| ATP + 5-deazariboflavin | 15% of the activity with riboflavin | Megasphaera elsdenii | ADP + 5-deazariboflavin 5'-phosphate | - |
? | |
| ATP + riboflavin | - |
Megasphaera elsdenii | ADP + FMN | - |
? | |
| GTP + riboflavin | no activity | Megasphaera elsdenii | GDP + riboflavin 5'-phosphate | - |
? | |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 45 | the relative activities at pH 7.2 and 20°C, 25°C, 37°C and 45°C are 0.22, 0.46, 1, and 1.35, respectively | Megasphaera elsdenii |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
