Inhibitors | Comment | Organism | Structure |
---|---|---|---|
7,8-dimethyl-10-(2'-hydroxyethyl)-isoalloxazine | 0.5 mM, 10% inhibition | Bacillus subtilis | |
7,8-dimethyl-10-(O-methylacetoxime)-isoaloxazine | 0.5 mM, 34% inhibition | Bacillus subtilis | |
Ca2+ | - |
Bacillus subtilis | |
Co2+ | - |
Bacillus subtilis | |
Lumiflavin | 0.5 mM, 4% inhibition | Bacillus subtilis | |
roseoflavin | 0.4 mM, 8% inhibition | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.112 | - |
ATP | pH 8, 37°C | Bacillus subtilis | |
0.18 | - |
riboflavin | pH 8, 37°C | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for maximal activity | Bacillus subtilis | |
Zn2+ | less effective in activation than Mg2+ | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27250 | - |
gel filtration | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + riboflavin | Bacillus subtilis | key enzyme in flavin biosynthesis | ADP + FMN | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
monofunctional enzyme | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + riboflavin | - |
Bacillus subtilis | ADP + FMN | - |
? | |
ATP + riboflavin | key enzyme in flavin biosynthesis | Bacillus subtilis | ADP + FMN | - |
? | |
CTP + riboflavin | 50% of the activity with ATP | Bacillus subtilis | CDP + riboflavin 5'-phosphate | - |
? | |
dATP + riboflavin | - |
Bacillus subtilis | dADP + FMN | - |
? | |
UTP + riboflavin | 31% of the activity with ATP | Bacillus subtilis | UDP + FMN | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
52 | - |
- |
Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Bacillus subtilis |