Any feedback?
Literature summary for 2.7.1.29 extracted from
- Cloning and overexpression in Escherichia coli of the gene encoding dihydroxyacetone kinase isoenzyme I from Schizosaccharomyces pombe, and its application to dihydroxyacetone phosphate production (1999), Appl. Microbiol. Biotechnol., 51, 193-200.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of dak 1 in Escherichia coli | Schizosaccharomyces pombe |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | competitive inhibition | Schizosaccharomyces pombe |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
dihydroxyacetone | pH 7.5, 25°C, recombinant enzyme | Schizosaccharomyces pombe | |
| 0.63 | - |
ATP | pH 7.5, 25°C, recombinant enzyme | Schizosaccharomyces pombe | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 4 mM, 116% activation | Schizosaccharomyces pombe | |
| Co2+ | 4 mM, 20% activation | Schizosaccharomyces pombe | |
| Mg2+ | 4 mM, 100% activation | Schizosaccharomyces pombe | |
| Mn2+ | 4 mM, 12% activation | Schizosaccharomyces pombe | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
2 * 62000, recombinant enzyme, SDS-PAGE | Schizosaccharomyces pombe |
| 62245 | - |
2 * 62245, recombinant enzyme, deduced from amino acid sequence | Schizosaccharomyces pombe |
| 145000 | - |
gel filtration | Schizosaccharomyces pombe |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schizosaccharomyces pombe | O13902 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, butyl-Toyopearl, UNO-Q6, recombinant enzyme | Schizosaccharomyces pombe |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.9 | - |
- |
Schizosaccharomyces pombe |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + dihydroxyacetone | - |
Schizosaccharomyces pombe | ADP + dihydroxyacetone phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 62000, recombinant enzyme, SDS-PAGE | Schizosaccharomyces pombe |
| dimer | 2 * 62245, recombinant enzyme, deduced from amino acid sequence | Schizosaccharomyces pombe |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
ADP | - |
Schizosaccharomyces pombe | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
