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Literature summary for 2.7.1.3 extracted from
- Bovine liver fructokinase: purification and kinetic properties (1977), Biochemistry, 16, 2169-2175.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-deoxy-D-fructose | dead-end inhibitor | Bos taurus |  |
| 2,5-anhydro-D-glucitol | - |
Bos taurus | |
| 2,5-anhydro-D-lyxitol | - |
Bos taurus | |
| 2,5-anhydro-D-mannitol | - |
Bos taurus | |
| 2,5-anhydro-D-mannose | - |
Bos taurus | |
| 5-Thio-D-fructose | competitive to D-fructose | Bos taurus | |
| CrADP | dead-end inhibitor | Bos taurus | |
| CrATP | dead-end inhibitor | Bos taurus | |
| D-Fructose 1-phosphate | competitive against D-fructose, noncompetitive to MgATP2- | Bos taurus | |
| D-mannoheptulose | - |
Bos taurus | |
| D-psicose | - |
Bos taurus | |
| D-ribono-gamma-lactone | - |
Bos taurus | |
| D-ribose | - |
Bos taurus | |
| D-sedoheptulose | - |
Bos taurus | |
| D-tagatose | - |
Bos taurus | |
| D-xylulose | - |
Bos taurus | |
| L-galactoheptulose | - |
Bos taurus | |
| L-ribulose | - |
Bos taurus | |
| L-sorbose | - |
Bos taurus | |
| MgADP- | competitive to MgATP2- | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
D-fructose | 25°C, pH 7 | Bos taurus | |
| 0.37 | - |
L-ribulose | 25°C, pH 7 | Bos taurus | |
| 0.43 | - |
L-sorbose | 25°C, pH 7 | Bos taurus | |
| 0.9 | - |
D-tagatose | 25°C, pH 7 | Bos taurus | |
| 1 | - |
2,5-anhydro-D-mannose | 25°C, pH 7 | Bos taurus | |
| 1.2 | - |
5-keto-D-fructose | 25°C, pH 7 | Bos taurus | |
| 1.4 | - |
D-xylose | 25°C, pH 7 | Bos taurus | |
| 1.6 | - |
2,5-anhydro-D-mannitol | 25°C, pH 7 | Bos taurus | |
| 1.8 | - |
L-galacto-2-heptulose | 25°C, pH 7, D-fructose 1-phosphate | Bos taurus | |
| 2.2 | - |
D-sedoheptulose | 25°C, pH 7 | Bos taurus | |
| 2.9 | - |
2,5-anhydro-D-glucitol | 25°C, pH 7 | Bos taurus | |
| 10 | - |
2,5-anhydro-D-lyxitol | 25°C, pH 7 | Bos taurus | |
| 11 | - |
D-psicose | 25°C, pH 7 | Bos taurus | |
| 58 | - |
D-ribono-gamma-lactone | 25°C, pH 7 | Bos taurus | |
| 120 | - |
D-mannoheptulose | 25°C, pH 7 | Bos taurus | |
| 142 | - |
D-ribose | 25°C, pH 7 | Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cs+ | activates | Bos taurus | |
| K+ | Km: 15 mM | Bos taurus | |
| Na+ | activates | Bos taurus | |
| NH4+ | activates | Bos taurus | |
| Rb+ | activates | Bos taurus | |
| Tl+ | activates | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
2 * 29000 SDS-PAGE in the presence of dithiothreitol | Bos taurus |
| 56000 | - |
gel filtration, ultracentrifugation | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose | Bos taurus | - |
ADP + D-fructose 1-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + D-fructose = ADP + D-fructose 1-phosphate | random kinetic mechanism with the formation of two dead-end complexes | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 17 | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2,5-anhydro-D-glucitol | - |
Bos taurus | ? | - |
r | |
| ATP + 2,5-anhydro-D-lyxitol | - |
Bos taurus | ? | - |
r | |
| ATP + 2,5-anhydro-D-mannitol | - |
Bos taurus | ? | - |
r | |
| ATP + 2,5-anhydro-D-mannose | - |
Bos taurus | ? | - |
r | |
| ATP + 5-keto-D-fructose | - |
Bos taurus | ? | - |
? | |
| ATP + D-fructose | - |
Bos taurus | ADP + D-fructose 1-phosphate | - |
r | |
| ATP + D-psicose | - |
Bos taurus | ? | - |
r | |
| ATP + D-ribono-gamma-lactone | - |
Bos taurus | ? | - |
r | |
| ATP + D-ribose | - |
Bos taurus | ? | - |
r | |
| ATP + D-sedoheptulose | - |
Bos taurus | ? | - |
r | |
| ATP + D-tagatose | - |
Bos taurus | ? | - |
r | |
| ATP + D-xylose | - |
Bos taurus | ADP + D-xylose 1-phosphate | - |
? | |
| ATP + D-xylulose | - |
Bos taurus | ? | - |
r | |
| ATP + L-galactoheptulose | - |
Bos taurus | ? | - |
r | |
| ATP + L-ribulose | - |
Bos taurus | ? | - |
r | |
| ATP + L-sorbose | - |
Bos taurus | ? | - |
r | |
| ATP + mannoheptulose | - |
Bos taurus | ? | - |
r | |
| additional information | the minimum molecule for substrate activity seems to be (2R)-2-hydroxymethyl-3,4-dihydroxytetrahydrofuran | Bos taurus | ? | - |
? | |
| additional information | not: 5-thio-D-fructose | Bos taurus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 29000 SDS-PAGE in the presence of dithiothreitol | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Bos taurus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bos taurus | isoelectric focusing, LKB (pH 3-10) ampholytes were used with 7.5% polyacrylamide gels | - |
5.7 |
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