Any feedback?
Literature summary for 2.7.1.30 extracted from
- Purification and properties of glycerokinase from Bacillus stearothermophilus (1979), J. Appl. Biochem., 1, 259-270.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fructose 1,6-diphosphate | - |
Escherichia coli |  |
| fructose 1,6-diphosphate | - |
Geobacillus stearothermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Escherichia coli | |
| additional information | - |
additional information | - |
Geobacillus stearothermophilus | |
| additional information | - |
additional information | - |
Candida mycoderma | |
| 0.15 | - |
glyceric acid | - |
Escherichia coli | |
| 0.15 | - |
glyceric acid | - |
Geobacillus stearothermophilus | |
| 0.15 | - |
glyceric acid | - |
Candida mycoderma | |
| 4.9 | - |
monothioglycerol | - |
Escherichia coli | |
| 4.9 | - |
monothioglycerol | - |
Candida mycoderma | |
| 4.9 | - |
monothioglycerol | 30°C | Geobacillus stearothermophilus | |
| 42 | - |
L-glyceraldehyde | - |
Escherichia coli | |
| 42 | - |
L-glyceraldehyde | - |
Candida mycoderma | |
| 42 | - |
L-glyceraldehyde | 30°C | Geobacillus stearothermophilus | |
| 100 | - |
dihydroxyacetone | - |
Escherichia coli | |
| 100 | - |
dihydroxyacetone | - |
Candida mycoderma | |
| 100 | - |
dihydroxyacetone | 30°C | Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
4 * 58000, SDS-PAGE | Geobacillus stearothermophilus |
| 230000 | - |
gel filtration | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida mycoderma | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Geobacillus stearothermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 116 | - |
30°C | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + dihydroxyacetone | - |
Escherichia coli | ADP + dihydroxyacetone phosphate | - |
? | |
| ATP + dihydroxyacetone | - |
Geobacillus stearothermophilus | ADP + dihydroxyacetone phosphate | - |
? | |
| ATP + dihydroxyacetone | - |
Candida mycoderma | ADP + dihydroxyacetone phosphate | - |
? | |
| ATP + glyceric acid | - |
Geobacillus stearothermophilus | ADP + ? | - |
? | |
| ATP + glyceric acid | - |
Escherichia coli | ? | - |
? | |
| ATP + glyceric acid | - |
Candida mycoderma | ? | - |
? | |
| ATP + glycerol | - |
Escherichia coli | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | - |
Geobacillus stearothermophilus | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + glycerol | - |
Candida mycoderma | ADP + sn-glycerol 3-phosphate | - |
? | |
| ATP + L-glyceraldehyde | - |
Escherichia coli | ADP + L-glyceraldehyde 3-phosphate | - |
? | |
| ATP + L-glyceraldehyde | - |
Geobacillus stearothermophilus | ADP + L-glyceraldehyde 3-phosphate | - |
? | |
| ATP + L-glyceraldehyde | - |
Candida mycoderma | ADP + L-glyceraldehyde 3-phosphate | - |
? | |
| ATP + mercaptopropanediol | - |
Escherichia coli | 1-mercaptopropanediol 1-phosphate + ADP | - |
? | |
| ATP + mercaptopropanediol | - |
Candida mycoderma | 1-mercaptopropanediol 1-phosphate + ADP | - |
? | |
| ATP + monothioglycerol | - |
Geobacillus stearothermophilus | ADP + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Escherichia coli |
| tetramer | 4 * 58000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Geobacillus stearothermophilus |
| 65 | - |
- |
Geobacillus stearothermophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 80 | 30°C: about 50% of maximum activity, 80°C: about 65% of maximum activity | Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
no loss of activity detectable | Geobacillus stearothermophilus |
| 20 | - |
t1/2 8.6 days | Escherichia coli |
| 20 | - |
t1/2: 4.1 days | Candida mycoderma |
| 60 | - |
t1/2: 310 min | Geobacillus stearothermophilus |
| 60 | - |
t1/2: 0.72 min | Candida mycoderma |
| 60 | - |
t1/2: 4.5 min | Escherichia coli |
| 70 | - |
- |
Escherichia coli |
| 70 | - |
- |
Candida mycoderma |
| 70 | - |
t1/2: 3 min | Geobacillus stearothermophilus |
| 80 | - |
t1/2: 0.53 min | Geobacillus stearothermophilus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
