Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 (DE3 + pRARE2) cells | Trypanosoma brucei gambiense |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 30% (w/v) PEG 400 and 100 mM HEPES pH 7.0, at 20°C | Trypanosoma brucei gambiense |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
glycosome | - |
Trypanosoma brucei gambiense | 20015 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60400 | - |
x * 60400, calculated from amino acid sequence | Trypanosoma brucei gambiense |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei gambiense | D3KVM3 | - |
- |
Trypanosoma brucei gambiense IL2343 | D3KVM3 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography and Superdex 200 gel filtration | Trypanosoma brucei gambiense |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + sn-glycerol 3-phosphate | - |
Trypanosoma brucei gambiense | ATP + glycerol | - |
r | |
ADP + sn-glycerol 3-phosphate | - |
Trypanosoma brucei gambiense IL2343 | ATP + glycerol | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 60400, calculated from amino acid sequence | Trypanosoma brucei gambiense |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is essential for energy metabolism | Trypanosoma brucei gambiense |