Literature summary for 2.7.1.30 extracted from

Balogun, E.O.; Inaoka, D.K.; Kido, Y.; Shiba, T.; Nara, T.; Aoki, T.; Honma, T.; Tanaka, A.; Inoue, M.; Matsuoka, S.; Michels, P.A.; Harada, S.; Kita, K.
Overproduction, purification, crystallization and preliminary X-ray diffraction analysis of Trypanosoma brucei gambiense glycerol kinase (2010), Acta Crystallogr. Sect. F, 66, 304-308.

Search for more literature for 2.7.1.30

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli JM109 (DE3 + pRARE2) cells	Trypanosoma brucei gambiense

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 30% (w/v) PEG 400 and 100 mM HEPES pH 7.0, at 20°C	Trypanosoma brucei gambiense

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
glycosome	-	Trypanosoma brucei gambiense	20015	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60400	-	x * 60400, calculated from amino acid sequence	Trypanosoma brucei gambiense

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma brucei gambiense	D3KVM3	-	-
Trypanosoma brucei gambiense IL2343	D3KVM3	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography and Superdex 200 gel filtration	Trypanosoma brucei gambiense

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + sn-glycerol 3-phosphate	-	Trypanosoma brucei gambiense	ATP + glycerol	-	r
ADP + sn-glycerol 3-phosphate	-	Trypanosoma brucei gambiense IL2343	ATP + glycerol	-	r

Subunits

Subunits	Comment	Organism
?	x * 60400, calculated from amino acid sequence	Trypanosoma brucei gambiense

General Information

General Information	Comment	Organism
physiological function	the enzyme is essential for energy metabolism	Trypanosoma brucei gambiense

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Release 2023.1 (January 2023)