Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Shot BL21 star (DE3) cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D72V | the catalytic properties of the mutant differ little from those of the wild type enzyme. The mutant shows 14.76% expression compared to the wild type enzyme | Escherichia coli |
M271I | the mutant shows strongly increased Km for ATP and 30.75% expression compared to the wild type enzyme | Escherichia coli |
Q37P | the mutant shows strongly increased Km for ATP and 65.73% expression compared to the wild type enzyme | Escherichia coli |
V61L | the catalytic properties of the mutant differ little from those of the wild type enzyme. The mutant shows 12.71% expression compared to the wild type enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | substrate inhibition | Escherichia coli | |
cytosolic subunit of the glucose-specific phosphotransferase system | allosteric inhibition | Escherichia coli | |
D-fructose-1,6-bisphosphate | allosteric inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
ATP | mutant enzyme V61L, at pH 7.0 and 25°C | Escherichia coli | |
0.0078 | - |
ATP | wild type enzyme, at pH 7.0 and 25°C | Escherichia coli | |
0.009 | - |
ATP | mutant enzyme D72V, at pH 7.0 and 25°C | Escherichia coli | |
0.19 | - |
ATP | mutant enzyme Q37P, at pH 7.0 and 25°C | Escherichia coli | |
0.25 | - |
ATP | mutant enzyme M271I, at pH 7.0 and 25°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+-ATP-dependent | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
2 or 4 * 60000, in solution, the enzyme exists in a dimer-tetramer equilibrium | Escherichia coli |
158000 | - |
dimeric native enzyme, gel filtration | Escherichia coli |
224000 | - |
tetrameric native enzyme, gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
MG1655 (ATCC 47076) | - |
Purification (Comment) | Organism |
---|---|
GSTrap FF column chromatography, gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycerol | - |
Escherichia coli | ADP + sn-glycerol 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer or tetramer | 2 or 4 * 60000, in solution, the enzyme exists in a dimer-tetramer equilibrium | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
GLPK | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | Mg2+-ATP-dependent | Escherichia coli |