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Literature summary for 2.7.1.30 extracted from

  • Ohashi-Suzuki, M.; Yabu, Y.; Ohshima, S.; Nakamura, K.; Kido, Y.; Sakamoto, K.; Kita, K.; Ohta, N.; Suzuki, T.
    Differential kinetic activities of glycerol kinase among African trypanosome species: phylogenetic and therapeutic implications (2011), J. Vet. Med. Sci., 73, 615-621.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Trypanosoma brucei brucei
expressed in Escherichia coli Trypanosoma vivax
expressed in Escherichia coli Trypanosoma congolense

Inhibitors

Inhibitors Comment Organism Structure
glycerol inhibitor of the glycerol kinase reverse reaction Trypanosoma brucei brucei
glycerol inhibitor of the glycerol kinase reverse reaction Trypanosoma congolense
glycerol inhibitor of the glycerol kinase reverse reaction Trypanosoma vivax

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
ADP in 0.1 M MOPS buffer (pH 6.8) containing 1 mM EDTA, 5 mM MgSO4, temperature not specified in the publication Trypanosoma congolense
0.37
-
ADP in 0.1 M MOPS buffer (pH 6.8) containing 1 mM EDTA, 5 mM MgSO4, temperature not specified in the publication Trypanosoma brucei brucei
0.69
-
ADP in 0.1 M MOPS buffer (pH 6.8) containing 1 mM EDTA, 5 mM MgSO4, temperature not specified in the publication Trypanosoma vivax
1.09
-
sn-glycerol 3-phosphate in 0.1 M MOPS buffer (pH 6.8) containing 1 mM EDTA, 5 mM MgSO4, temperature not specified in the publication Trypanosoma congolense
1.13
-
sn-glycerol 3-phosphate in 0.1 M MOPS buffer (pH 6.8) containing 1 mM EDTA, 5 mM MgSO4, temperature not specified in the publication Trypanosoma brucei brucei
1.37
-
sn-glycerol 3-phosphate in 0.1 M MOPS buffer (pH 6.8) containing 1 mM EDTA, 5 mM MgSO4, temperature not specified in the publication Trypanosoma vivax

Localization

Localization Comment Organism GeneOntology No. Textmining
glycosome
-
Trypanosoma brucei brucei 20015
-
glycosome
-
Trypanosoma vivax 20015
-
glycosome
-
Trypanosoma congolense 20015
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
x * 50000, SDS-PAGE Trypanosoma brucei brucei
50000
-
x * 50000, SDS-PAGE Trypanosoma vivax
50000
-
x * 50000, SDS-PAGE Trypanosoma congolense
54600
-
x * 54600, calculated from amino acid sequence Trypanosoma vivax
56200
-
x * 56200, calculated from amino acid sequence Trypanosoma congolense

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei brucei Q9NJP9
-
-
Trypanosoma brucei brucei ILTat1.4 Q9NJP9
-
-
Trypanosoma congolense Q75T26
-
-
Trypanosoma congolense IL1180 Q75T26
-
-
Trypanosoma vivax B0I530
-
-
Trypanosoma vivax IL1392 B0I530
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Trypanosoma brucei brucei
nickel affinity column chromatography Trypanosoma vivax
nickel affinity column chromatography Trypanosoma congolense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + sn-glycerol 3-phosphate
-
Trypanosoma brucei brucei ATP + glycerol
-
r
ADP + sn-glycerol 3-phosphate
-
Trypanosoma vivax ATP + glycerol
-
r
ADP + sn-glycerol 3-phosphate
-
Trypanosoma congolense ATP + glycerol
-
r
ADP + sn-glycerol 3-phosphate
-
Trypanosoma congolense IL1180 ATP + glycerol
-
r
ADP + sn-glycerol 3-phosphate
-
Trypanosoma brucei brucei ILTat1.4 ATP + glycerol
-
r
ADP + sn-glycerol 3-phosphate
-
Trypanosoma vivax IL1392 ATP + glycerol
-
r
ATP + glycerol
-
Trypanosoma brucei brucei ADP + sn-glycerol 3-phosphate
-
r
ATP + glycerol
-
Trypanosoma vivax ADP + sn-glycerol 3-phosphate
-
r
ATP + glycerol
-
Trypanosoma congolense ADP + sn-glycerol 3-phosphate
-
r
ATP + glycerol
-
Trypanosoma congolense IL1180 ADP + sn-glycerol 3-phosphate
-
r
ATP + glycerol
-
Trypanosoma brucei brucei ILTat1.4 ADP + sn-glycerol 3-phosphate
-
r
ATP + glycerol
-
Trypanosoma vivax IL1392 ADP + sn-glycerol 3-phosphate
-
r

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Trypanosoma brucei brucei
? x * 50000, SDS-PAGE Trypanosoma vivax
? x * 50000, SDS-PAGE Trypanosoma congolense
? x * 56200, calculated from amino acid sequence Trypanosoma congolense
? x * 54600, calculated from amino acid sequence Trypanosoma vivax