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Literature summary for 2.7.1.32 extracted from
- Structure and function of choline kinase isoforms in mammalian cells (2004), Prog. Lipid Res., 43, 266-281.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| parts of the alpha and beta subtype expressed as GST-fusion protein in Escherichia coli, alpha and beta subtype coexpressed in COS-7 cells | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
choline | dimer | Caenorhabditis elegans |  |
| 0.013 | - |
choline | liver enzyme | Rattus norvegicus | |
| 0.014 | - |
choline | brain enzyme | Rattus norvegicus | |
| 0.04 | - |
ATP | liver enzyme | Rattus norvegicus | |
| 0.09 | - |
ATP | - |
Saccharomyces cerevisiae | |
| 0.1 | - |
choline | kidney enzyme | Rattus norvegicus | |
| 0.27 | - |
choline | - |
Saccharomyces cerevisiae | |
| 1 | - |
ATP | brain enzyme | Rattus norvegicus | |
| 1.5 | - |
ATP | kidney enzyme | Rattus norvegicus | |
| 2.4 | - |
ATP | dimer | Caenorhabditis elegans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Mus musculus | |
| Mg2+ | required | Homo sapiens | |
| Mg2+ | required | Rattus norvegicus | |
| Mg2+ | required | Saccharomyces cerevisiae | |
| Mg2+ | required | Caenorhabditis elegans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
2 * 50000-52000, different molecular weights for splicing variants of the alpha subtype, 20% of enzyme activity, 2 * 45000, beta subtype, 20% of enzyme activity, heterodimer of both subtypes, 1 * 50000-52000 + 1 * 45000, 60% of enzyme activity | Mus musculus |
| 45000 | - |
enzymes from kidney and brain, 2 * 45000, kidney enzyme, beta subtype | Rattus norvegicus |
| 52000 | - |
2 * 52000, glioblastoma enzyme, alpha subtype, homo- or heterodimers formed from alpha and beta subtype | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + choline | Saccharomyces cerevisiae | initial step of the CDP-choline pathway, that yields phosphatidylcholine as final product, involved in mitogenic signaling transduction | ADP + O-phosphocholine | - |
? | |
| ATP + choline | Caenorhabditis elegans | initial step of the CDP-choline pathway, that yields phosphatidylcholine as final product, involved in mitogenic signaling transduction | ADP + O-phosphocholine | - |
? | |
| ATP + choline | Rattus norvegicus | initial step of the CDP-choline pathway, that yields phosphatidylcholine as final product, involved in mitogenic signaling transduction and cell stress regulation | ADP + O-phosphocholine | - |
? | |
| ATP + choline | Mus musculus | initial step of the CDP-choline pathway, that yields phosphatidylcholine as final product, involved in mitogenic signaling transduction, involved in cell stress response | ADP + O-phosphocholine | - |
? | |
| ATP + choline | Homo sapiens | initial step of the CDP-choline pathway, that yields phosphatidylcholine as final product, involved in mitogenic signaling transduction, possible role in carcinogenesis | ADP + O-phosphocholine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caenorhabditis elegans | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
embryo | - |
| Rattus norvegicus | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant proteins using GST-tag | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| glioblastoma cell | - |
Homo sapiens | - |
| heart | high expression of the beta subtype | Mus musculus | - |
| hepatocyte | - |
Mus musculus | - |
| kidney | subtype beta | Rattus norvegicus | - |
| liver | high expression of the alpha and beta subtype | Mus musculus | - |
| liver | subtype alpha | Rattus norvegicus | - |
| testis | high expression of the alpha subtype | Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + choline | - |
Mus musculus | ADP + O-phosphocholine | - |
? | |
| ATP + choline | - |
Homo sapiens | ADP + O-phosphocholine | - |
? | |
| ATP + choline | - |
Rattus norvegicus | ADP + O-phosphocholine | - |
? | |
| ATP + choline | - |
Saccharomyces cerevisiae | ADP + O-phosphocholine | - |
? | |
| ATP + choline | - |
Caenorhabditis elegans | ADP + O-phosphocholine | - |
? | |
| ATP + choline | initial step of the CDP-choline pathway, that yields phosphatidylcholine as final product, involved in mitogenic signaling transduction | Saccharomyces cerevisiae | ADP + O-phosphocholine | - |
? | |
| ATP + choline | initial step of the CDP-choline pathway, that yields phosphatidylcholine as final product, involved in mitogenic signaling transduction | Caenorhabditis elegans | ADP + O-phosphocholine | - |
? | |
| ATP + choline | initial step of the CDP-choline pathway, that yields phosphatidylcholine as final product, involved in mitogenic signaling transduction and cell stress regulation | Rattus norvegicus | ADP + O-phosphocholine | - |
? | |
| ATP + choline | initial step of the CDP-choline pathway, that yields phosphatidylcholine as final product, involved in mitogenic signaling transduction, involved in cell stress response | Mus musculus | ADP + O-phosphocholine | - |
? | |
| ATP + choline | initial step of the CDP-choline pathway, that yields phosphatidylcholine as final product, involved in mitogenic signaling transduction, possible role in carcinogenesis | Homo sapiens | ADP + O-phosphocholine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 50000-52000, different molecular weights for splicing variants of the alpha subtype, 20% of enzyme activity, 2 * 45000, beta subtype, 20% of enzyme activity, heterodimer of both subtypes, 1 * 50000-52000 + 1 * 45000, 60% of enzyme activity | Mus musculus |
| dimer | 2 * 52000, glioblastoma enzyme, alpha subtype, homo- or heterodimers formed from alpha and beta subtype | Homo sapiens |
| dimer | enzymes from kidney and brain, 2 * 45000, kidney enzyme, beta subtype | Rattus norvegicus |
| oligomer | dimers, tetramers and octamers | Saccharomyces cerevisiae |
| oligomer | mainly dimers | Caenorhabditis elegans |
| tetramer | enzyme from liver, 4 * 50000-52000, different molecular weights for splicing variants of the alpha subtype | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | alpha and beta subtype | Mus musculus |
| More | alpha and beta subtype | Homo sapiens |
| More | alpha and beta subtype | Rattus norvegicus |
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Release 2023.1 (January 2023)
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