Application | Comment | Organism |
---|---|---|
diagnostics | prognostic factor in human cancer | Homo sapiens |
drug development | target for therapy since its specific inhibitors display efficient antitumoral activity in vivo | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
Transfection of human HeK293T is carried out by the calcium phosphate method. The amount of plasmidic DNA is kept constant at 0.2 mg with the corresponding empty vector. Tumors induced by ChoK alpha over-expression in Hek293T are removed from the mice to generate the ChoK tumoral cell line ADJ. Different slices of the tissue are placed into culture 100mm dishes and treated with trypsin and G418 to avoid murine cells contamination. The resultant cell line (ADJ) displays constitutive ChoK alpha activation and a fully transformed phenotype | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293T cell | Transfection of human HeK293T is carried out by the calcium phosphate method. The amount of plasmidic DNA is kept constant at 2 microg with the corresponding empty vector. Tumors induced by ChoK alpha over-expression in Hek293T are removed from the mice to generate the ChoK tumoral cell line ADJ. Different slices of the tissue are placed into culture 100mm dishes and treated with trypsin and 1 mg/ml of G418 to avoid murine cells contamination. The resultant cell line ADJ displays constitutive ChoK alpha activation and a fully transformed phenotype | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
Genes significantly down-regulated by choline kinase: BCL2-interacting killer (apoptosis-inducing), stabilization of the calcium channel, cyclin G2. Inhibition of cell proliferation, T-cell surface protein tactile, cyclin-dependent kinase inhibitor 1A (p21, Cip1), cyclin-dependent kinase inhibitor 1B (p27, Kip1), dual-specificity-(Y)-tyrosine phosphor regulated kinase, Mad signalling (altered response to quimiotherapy), opioid receptor, mu 1 (negative regulat of cell proliferation), retinoblastoma-like 1 (p107) (Rb associated protein), transforming growth factor, beta receptor I, Toll like receptor (antitumoral immunity), immune escape of human cancer, Visinin like 1 | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ChoK | - |
Homo sapiens |
ChoK alpha | - |
Homo sapiens |
choline kinase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |