General Stability | Organism |
---|---|
ATP and/or sucrose stabilize during purification | Rattus norvegicus |
considerably unstable independent of the degree of purity | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-Ketopantetheine | - |
Rattus norvegicus | |
CoA | strong | Rattus norvegicus | |
dephospho-CoA | - |
Rattus norvegicus | |
L-Pantothenate | - |
Rattus norvegicus | |
pantetheine 4'-phosphate | strong | Rattus norvegicus | |
pantothenoylcysteine 4'-phosphate | strong | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
pantothenate | pH 6.1, 37°C | Rattus norvegicus | |
1 | - |
ATP | pH 6.1, 37°C | Rattus norvegicus | |
1 | - |
ATP | ATP in form of MgATP2- | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement, active substrate: MgATP-complex | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-pantothenate | Rattus norvegicus | first and rate-controlling reaction of CoA-biosynthesis | ADP + D-4'-phosphopantothenate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male Wistar | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0041 | - |
pH 6.1, 37°C | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, above 3 mg protein/ml, more than 1 week | Rattus norvegicus |
4°C, above 3 mg protein/ml, 4 days | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantothenate | - |
Rattus norvegicus | ADP + (R)-4'-phosphopantothenate | - |
? | |
ATP + D-pantothenate | D-configuration of 2'-hydroxyl group in pantothenate molecule is essential for functional interaction with enzyme | Rattus norvegicus | ADP + D-4'-phosphopantothenate | - |
? | |
ATP + D-pantothenate | first and rate-controlling reaction of CoA-biosynthesis | Rattus norvegicus | ADP + D-4'-phosphopantothenate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.9 | 6.3 | - |
Rattus norvegicus |