Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.33 extracted from

  • Zhang, Y.M.; Rock, C.O.; Jackowski, S.
    Feedback regulation of murine pantothenate kinase 3 by coenzyme A and coenzyme A thioesters (2005), J. Biol. Chem., 280, 32594-32601.
    View publication on PubMed
Search for more literature for 2.7.1.33

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis of isozymes PanK3 and PanK1beta, overexpression of wild-type isozymes PanK3 and PanK1beta and chimeric mutants thereof in HEK293T cells Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information construction of chimeric mutant enzymes PanK1beta-3-1beta and PanK3-1beta-3 by combination of isozymes PanK3 and PanK1beta, mutant show different sensitivity to feedback inhibitors compared to the wild-type isozymes, overview Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
acetyl-CoA feedback inhibition, regulatory function, isozyme PanK1beta and isozyme PanK3, strong inhibition of mutant chimera PanK1beta-3-1beta, slight inhibition of mutant chimera PanK3-1beta-3 Mus musculus
coenzyme A feedback inhibition, regulatory function, isozyme PanK1beta, slight inhibition of isozyme PanK3 Mus musculus
malonyl-CoA feedback inhibition, regulatory function, isozyme PanK3, slight inhibition of isozyme PanK1beta Mus musculus
additional information structural determinants for feedback inhibition Mus musculus
palmitoyl-CoA feedback inhibition, regulatory function, isozyme PanK3, mutant chimera PanK1beta-3-1beta, slight inhibition of mutant chimera PanK3-1beta-3 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0057
-
 (R)-pantothenate recombinant isozyme PanK1beta, pH 7.0, 37°C Mus musculus
0.0095
-
 (R)-pantothenate recombinant isozyme PanK3, pH 7.0, 37°C Mus musculus
0.087
-
 ATP recombinant isozyme PanK1beta, pH 7.0, 37°C Mus musculus
0.112
-
 ATP recombinant isozyme PanK3, pH 7.0, 37°C Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41100
-
 2 * 41100, isozyme Pank3, sequence calculation and gel filtration, 2 * 41600, isozyme Pank1beta, sequence calculation and gel filtration Mus musculus
41600
-
 2 * 41100, isozyme Pank3, sequence calculation and gel filtration, 2 * 41600, isozyme Pank1beta, sequence calculation and gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-pantothenate Mus musculus first step in coenzyme A biosynthesis, regulatory function ADP + (R)-4'-phosphopantothenate
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 male C57BL6/J mice, isozyme PanK3 and PanK1beta
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-pantothenate
-
 Mus musculus ADP + (R)-4'-phosphopantothenate
-
 ?
ATP + (R)-pantothenate first step in coenzyme A biosynthesis, regulatory function Mus musculus ADP + (R)-4'-phosphopantothenate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 41100, isozyme Pank3, sequence calculation and gel filtration, 2 * 41600, isozyme Pank1beta, sequence calculation and gel filtration Mus musculus

Synonyms

Synonyms Comment Organism
PanK
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mus musculus