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Literature summary for 2.7.1.33 extracted from

  • Yang, K.; Eyobo, Y.; Brand, L.A.; Martynowski, D.; Tomchick, D.; Strauss, E.; Zhang, H.
    Crystal structure of a type III pantothenate kinase: insight into the mechanism of an essential coenzyme A biosynthetic enzyme universally distributed in bacteria (2006), J. Bacteriol., 188, 5532-5540.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.33

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(DE3) Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion method with 15% polyethylene glycol 3350 Thermotoga maritima

Protein Variants

Protein Variants Comment Organism
D105E less than 6% activity of the wild type enzyme Thermotoga maritima
D105N less than 6% activity of the wild type enzyme Thermotoga maritima
D125E less than 6% activity of the wild type enzyme Thermotoga maritima
D125N less than 6% activity of the wild type enzyme Thermotoga maritima
D6E less than 6% activity of the wild type enzyme Thermotoga maritima
D6N less than 6% activity of the wild type enzyme Thermotoga maritima

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by CoA or its thioesters Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
 (R)-pantothenate in 100 mM HEPES, pH 7.6, 20 mM KCl, 10 mM MgCl2, 2 mM phosphoenolpyruvate, 0.3 mM NADH, 5 units of lactate dehydrogenase, 2.5 units of pyruvate kinase, and 0.00027 mM of the PanK-III protein, at 50°C Thermotoga maritima
6.04
-
 ATP in 100 mM HEPES, pH 7.6, 20 mM KCl, 10 mM MgCl2, 2 mM phosphoenolpyruvate, 0.3 mM NADH, 5 units of lactate dehydrogenase, 2.5 units of pyruvate kinase, and 0.00027 mM of the PanK-III protein, at 50°C Thermotoga maritima

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-nitrilotriacetic acid-agarose column chromatography and Resource Q column chromatography Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-pantothenate
-
 Thermotoga maritima ADP + (R)-4'-phosphopantothenate
-
 ?

Subunits

Subunits Comment Organism
dimer gel filtration, functional unit in solution Thermotoga maritima
hexamer X-ray crystallography, in the crystal form Thermotoga maritima

Synonyms

Synonyms Comment Organism
PanK-III
-
 Thermotoga maritima
type III pantothenate kinase
-
 Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.29
-
 (R)-pantothenate in 100 mM HEPES, pH 7.6, 20 mM KCl, 10 mM MgCl2, 2 mM phosphoenolpyruvate, 0.3 mM NADH, 5 units of lactate dehydrogenase, 2.5 units of pyruvate kinase, and 0.00027 mM of the PanK-III protein, at 50°C Thermotoga maritima

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Thermotoga maritima